Mouse Gck ELISA Kit

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  • Alternative name

    Mouse hexokinase type IV ELISA Kit;Mouse HK IV ELISA Kit;Mouse hexokinase-4 ELISA Kit;Mouse HK4 ELISA Kit;Mouse hexokinase-D ELISA Kit;Mouse Gk ELISA Kit;Mouse GLK ELISA Kit;Mouse Gls006 ELISA Kit;Mouse HKIV ELISA Kit;Mouse HXKP ELISA Kit;Mouse Hlb62 ELISA Kit;Mouse MODY2 ELISA Kit;Mouse glucokinase ELISA Kit;

  • Catalog
    E054026
  • species
    Mouse
  • GeneGck
  • Other Species Human GCK ELISA KitRat Gck ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse GCK. No significant cross-reactivity or interference between Mouse GCK and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse GCK and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.094ng/mL
  • Intended UseMouse Gck ELISA Kit allows for the in vitro quantitative determination of Gck , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GCK in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GCK. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse GCK and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GCK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse GCK. You can calculate the concentration of Mouse GCK in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Glucokinase Protein information
  • Uniprot ID HXK4_MOUSE
  • Uniprot AC P52792; P52791;
  • UniGene Mm.220358;
  • GeneID 103988
  • KEGG mmu:103988;
  • Mouse Glucokinase Protein SEQUENCE
  • SEQUENCE 465 AA; 52089 MW; 8C85EED079A52D61 CRC64;

    MLDDRARMEA TKKEKVEQIL AEFQLQEEDL KKVMSRMQKE MDRGLKLETH

    QEASVKMLPT YVRSTPEGSE VGDFLSLDLG GTNFRVMLVK VGEGEAGQWS

    VKTKHQMYSI PEDAMTGTAE MLFDYISECI SDFLDKHQMK HKKLPLGFTF

    SFPVRHEDID KGILLNWTKG FKASGAEGNN IVGLLRDAIK RRGDFEMDVV

    AMVNDTVATM ISCYYEDRQC EVGMIVGTGC NACYMEEMQN VELVEGDEGR

    MCVNTEWGAF GNSGELDEFL LEYDRMVDES SVNPGQQLYE KIIGGKYMGE

    LVRLVLLKLV EENLLFHGEA SEQLRTRGAF ETRFVSQVES DSGDRRQILN

    ILSTLGLRPS VADCDIVRRA CESVSTRAAH MCSAGLAGVI NRMRESRSED

    VMRITVGVDG SVYKLHPSFK ERFHASVRRL TPNCEITFIE SEEGSGRGAA

    LVSAVACKKA CMLGQ

  • UCSC uc007hxn.2; mouse. [P52792-1];



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