Mouse Gja5 ELISA Kit

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  • Alternative name

    Mouse connexin-40 ELISA Kit;Mouse Cx40 ELISA Kit;Mouse Cxn-40 ELISA Kit;Mouse 5730555N10Rik ELISA Kit;Mouse Cnx40 ELISA Kit;Mouse Gja-5 ELISA Kit;Mouse gap junction protein, alpha 5 ELISA Kit;Mouse gap junction alpha-5 protein ELISA Kit;Mouse gap junction membrane channel protein alpha 5 ELISA Kit;

  • Catalog
    E053924
  • species
    Mouse
  • GeneGja5
  • Standard CurveMouse Gja5 ELISA Kit
  • Other Species Human GJA5 ELISA KitBovine GJA5 ELISA KitCanine GJA5 ELISA KitChicken GJA5 ELISA KitRat Gja5 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseMouse Gja5 ELISA Kit allows for the in vitro quantitative determination of Gja5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: This GJA5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GJA5. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: GJA5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GJA5 antibody and an GJA5-HRP conjugate. The assay sample and buffer are incubated together with GJA5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GJA5 concentration since GJA5 from samples and GJA5-HRP conjugate compete for the anti-GJA5 antibody binding site. Since the number of sites is limited, as more sites are occupied by GJA5 from the sample, fewer sites are left to bind GJA5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GJA5 concentration in each sample is interpolated from this standard curve.
  • Mouse Gap junction alpha-5 protein Protein information
  • Uniprot ID CXA5_MOUSE
  • Uniprot AC Q01231;
  • UniGene Mm.281816;
  • GeneID 14613
  • KEGG mmu:14613;
  • Mouse Gap junction alpha-5 protein Protein SEQUENCE
  • SEQUENCE 358 AA; 40413 MW; C5418F657142896A CRC64;

    MGDWSFLGEF LEEVHKHSTV IGKVWLTVLF IFRMLVLGTA AESSWGDEQA

    DFRCDTIQPG CQNVCYDQAF PISHIRYWVL QIIFVSTPSL VYMGHAMHTV

    RMQEKQKLRD AEKAKEAHRT GAYEYPVAEK AELSCWKEVD GKIVLQGTLL

    NTYVCTILIR TTMEVAFIVG QYLLYGIFLD TLHVCRRSPC PHPVNCYVSR

    PTEKNVFIVF MMAVAGLSLF LSLAELYHLG WKKIRQRFGK SRQGVDKHQL

    PGPPTSLVQS LTPPPDFNQC LKNSSGEKFF SDFSNNMGSR KNPDALATGE

    VPNQEQIPGE GFIHMHYSQK PEYASGASAG HRLPQGYHSD KRRLSKASSK

    ARSDDLSV

  • UCSC uc008qoo.2; mouse.;




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