Mouse Gjb1 ELISA Kit

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  • Alternative name

    Mouse connexin-32 ELISA Kit;Mouse Cx32 ELISA Kit;Mouse Cxn-32 ELISA Kit;Mouse Cnx32 ELISA Kit;Mouse gap junction protein, beta 1 ELISA Kit;Mouse gap junction beta-1 protein ELISA Kit;Mouse connexin 32 ELISA Kit;Mouse connexin-43 ELISA Kit;Mouse gap junction membrane channel protein beta 1 ELISA Kit;

  • Catalog
    E053920
  • species
    Mouse
  • GeneGjb1
  • Standard CurveMouse Gjb1 ELISA Kit
  • Other Species Human GJB1 ELISA KitBovine GJB1 ELISA KitHorse GJB1 ELISA KitMonkey GJB1 ELISA KitRat Gjb1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Gjb1 ELISA Kit allows for the in vitro quantitative determination of Gjb1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: GJB1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GJB1 antibody and an GJB1-HRP conjugate. The assay sample and buffer are incubated together with GJB1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GJB1 concentration since GJB1 from samples and GJB1-HRP conjugate compete for the anti-GJB1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GJB1 from the sample, fewer sites are left to bind GJB1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GJB1 concentration in each sample is interpolated from this standard curve.
  • Mouse Gap junction beta-1 protein Protein information
  • Uniprot ID CXB1_MOUSE
  • Uniprot AC P28230;
  • UniGene Mm.21198;
  • GeneID 14618
  • KEGG mmu:14618;
  • Mouse Gap junction beta-1 protein Protein SEQUENCE
  • SEQUENCE 283 AA; 32004 MW; C79FC46AA13BC5D7 CRC64;

    MNWTGLYTLL SGVNRHSTAI GRVWLSVIFI FRIMVLVVAA ESVWGDEKSS

    FICNTLQPGC NSVCYDHFFP ISHVRLWSLQ LILVSTPALL VAMHVAHQQH

    IEKKMLRLEG HGDPLHLEEV KRHKVHISGT LWWTYVISVV FRLLFEAVFM

    YVFYLLYPGY AMVRLVKCEA FPCPNTVDCF VSRPTEKTVF TVFMLAASGI

    CIILNVAEVV YLIIRACARR AQRRSNPPSR KGSGFGHRLS PEYKQNEINK

    LLSEQDGSLK DILRRSPGTG AGLAEKSDRC SAC

  • UCSC uc009txk.2; mouse.;


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