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Alternative name
Galectin-12 ELISA KIT; Galectin-related inhibitor of proliferationLGALS12 ELISA KIT; GRIP1 ELISA KIT; Gal-12 ELISA KIT
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Catalog
E053784
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species
Mouse
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GeneGAL12
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Galectin 12 (GAL12).
No significant cross-reactivity or interference between Galectin 12 (GAL12) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 14.5pg/mL.
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Intended UseMouse GAL12 ELISA Kit allows for the in vitro quantitative determination of GAL12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galectin 12 (GAL12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galectin 12 (GAL12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galectin 12 (GAL12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Galectin 12 (GAL12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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