Human BTK ELISA Kit

Alternative name
Human Agammaglobulinemia tyrosine kinase ELISA Kit;Human ATK ELISA Kit;Human B-cell progenitor kinase ELISA Kit;Human BPK ELISA Kit;Human Bruton tyrosine kinase ELISA Kit;Human AGMX1 ELISA Kit;Human AT ELISA Kit;Human IMD1 ELISA Kit;Human PSCTK1 ELISA Kit;Human XLA ELISA Kit;Human tyrosine-protein kinase BTK ELISA Kit;Human Bruton agammaglobulinemia tyrosine kinase ELISA Kit;Human Bruton's tyrosine kinase ELISA Kit;Human agammaglobulinaemia tyrosine kinase ELISA Kit;Human dominant-negative kinase-deficient Brutons tyrosine kinase ELISA Kit;Human truncated Bruton agammaglobulinemia tyrosine kinase ELISA Kit;Human tyrosine-protein kinase BTK isoform (lacking exon 13 to 17) ELISA Kit;Human tyrosine-protein kinase BTK isoform (lacking exon 14) ELISA Kit;
Catalog
E005364
species
Human
GeneBTK
Standard Curve
Other Species Mouse Btk ELISA Kit Chicken BTK ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman BTK ELISA Kit allows for the in vitro quantitative determination of BTK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This BTK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BTK. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: BTK ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for BTK. Standards or samples are then added to the microtiter plate wells and BTK if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of BTK present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for BTK are added to each well to "sandwich" the BTK immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain BTK and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BTK concentration in each sample is interpolated from this standard curve.
Human Tyrosine-protein kinase BTK Protein information
Uniprot ID BTK_HUMAN
Uniprot AC Q06187; B2RAW1; Q32ML5;
UniGene Hs.159494; Hs.733206;
GeneID 695
KEGG hsa:695;
Human Tyrosine-protein kinase BTK Protein SEQUENCE
SEQUENCE 659 AA; 76281 MW; DF06B5D1FEC257CC CRC64;
MAAVILESIF LKRSQQKKKT SPLNFKKRLF LLTVHKLSYY EYDFERGRRG
SKKGSIDVEK ITCVETVVPE KNPPPERQIP RRGEESSEME QISIIERFPY
PFQVVYDEGP LYVFSPTEEL RKRWIHQLKN VIRYNSDLVQ KYHPCFWIDG
QYLCCSQTAK NAMGCQILEN RNGSLKPGSS HRKTKKPLPP TPEEDQILKK
PLPPEPAAAP VSTSELKKVV ALYDYMPMNA NDLQLRKGDE YFILEESNLP
WWRARDKNGQ EGYIPSNYVT EAEDSIEMYE WYSKHMTRSQ AEQLLKQEGK
EGGFIVRDSS KAGKYTVSVF AKSTGDPQGV IRHYVVCSTP QSQYYLAEKH
LFSTIPELIN YHQHNSAGLI SRLKYPVSQQ NKNAPSTAGL GYGSWEIDPK
DLTFLKELGT GQFGVVKYGK WRGQYDVAIK MIKEGSMSED EFIEEAKVMM
NLSHEKLVQL YGVCTKQRPI FIITEYMANG CLLNYLREMR HRFQTQQLLE
MCKDVCEAME YLESKQFLHR DLAARNCLVN DQGVVKVSDF GLSRYVLDDE
YTSSVGSKFP VRWSPPEVLM YSKFSSKSDI WAFGVLMWEI YSLGKMPYER
FTNSETAEHI AQGLRLYRPH LASEKVYTIM YSCWHEKADE RPTFKILLSN
ILDVMDEES
UCSC uc004ehg.3; human. [Q06187-1];

Related products with Human BTK ELISA Kit

anti-BTK antibody

Category

Human BTK ELISA Kit