Human ACVB ELISA Kit

Contact Us
  • Catalog
    E000535
  • species
    Human
  • GeneACVB
  • Standard CurveHuman ACVB ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Activin B (ACVB). No significant cross-reactivity or interference between Activin B (ACVB) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 6.2pg/mL.
  • Intended UseHuman ACVB ELISA Kit allows for the in vitro quantitative determination of ACVB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activin B (ACVB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activin B (ACVB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activin B (ACVB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activin B (ACVB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


Related products with Human ACVB ELISA Kit


tag

Inquiry About Human ACVB ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!