Human BRD4 ELISA Kit

Alternative name
Human Protein HUNK1 ELISA Kit;Human HUNK1 ELISA Kit;Human CAP ELISA Kit;Human HUNKI ELISA Kit;Human MCAP ELISA Kit;Human bromodomain containing 4 ELISA Kit;Human bromodomain-containing protein 4 ELISA Kit;Human chromosome-associated protein ELISA Kit;
Catalog
E005340
species
Human
GeneBRD4
Other Species Mouse BRD4 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of human BRD4. No significant cross-reactivity or interference between human BRD4 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human BRD4 is typically less than 4.68 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman BRD4 ELISA Kit allows for the in vitro quantitative determination of BRD4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BRD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BRD4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BRD4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BRD4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Bromodomain-containing protein 4 Protein information
Uniprot ID BRD4_HUMAN
Uniprot AC O60885; O60433; Q4G0X8; Q86YS8; Q96PD3;
UniGene Hs.187763;
GeneID 23476
KEGG hsa:23476;
Human Bromodomain-containing protein 4 Protein SEQUENCE
SEQUENCE 1362 AA; 152219 MW; D52EFE1CF9960907 CRC64;
MSAESGPGTR LRNLPVMGDG LETSQMSTTQ AQAQPQPANA ASTNPPPPET
SNPNKPKRQT NQLQYLLRVV LKTLWKHQFA WPFQQPVDAV KLNLPDYYKI
IKTPMDMGTI KKRLENNYYW NAQECIQDFN TMFTNCYIYN KPGDDIVLMA
EALEKLFLQK INELPTEETE IMIVQAKGRG RGRKETGTAK PGVSTVPNTT
QASTPPQTQT PQPNPPPVQA TPHPFPAVTP DLIVQTPVMT VVPPQPLQTP
PPVPPQPQPP PAPAPQPVQS HPPIIAATPQ PVKTKKGVKR KADTTTPTTI
DPIHEPPSLP PEPKTTKLGQ RRESSRPVKP PKKDVPDSQQ HPAPEKSSKV
SEQLKCCSGI LKEMFAKKHA AYAWPFYKPV DVEALGLHDY CDIIKHPMDM
STIKSKLEAR EYRDAQEFGA DVRLMFSNCY KYNPPDHEVV AMARKLQDVF
EMRFAKMPDE PEEPVVAVSS PAVPPPTKVV APPSSSDSSS DSSSDSDSST
DDSEEERAQR LAELQEQLKA VHEQLAALSQ PQQNKPKKKE KDKKEKKKEK
HKRKEEVEEN KKSKAKEPPP KKTKKNNSSN SNVSKKEPAP MKSKPPPTYE
SEEEDKCKPM SYEEKRQLSL DINKLPGEKL GRVVHIIQSR EPSLKNSNPD
EIEIDFETLK PSTLRELERY VTSCLRKKRK PQAEKVDVIA GSSKMKGFSS
SESESSSESS SSDSEDSETE MAPKSKKKGH PGREQKKHHH HHHQQMQQAP
APVPQQPPPP PQQPPPPPPP QQQQQPPPPP PPPSMPQQAA PAMKSSPPPF
IATQVPVLEP QLPGSVFDPI GHFTQPILHL PQPELPPHLP QPPEHSTPPH
LNQHAVVSPP ALHNALPQQP SRPSNRAAAL PPKPARPPAV SPALTQTPLL
PQPPMAQPPQ VLLEDEEPPA PPLTSMQMQL YLQQLQKVQP PTPLLPSVKV
QSQPPPPLPP PPHPSVQQQL QQQPPPPPPP QPQPPPQQQH QPPPRPVHLQ
PMQFSTHIQQ PPPPQGQQPP HPPPGQQPPP PQPAKPQQVI QHHHSPRHHK
SDPYSTGHLR EAPSPLMIHS PQMSQFQSLT HQSPPQQNVQ PKKQELRAAS
VVQPQPLVVV KEEKIHSPII RSEPFSPSLR PEPPKHPESI KAPVHLPQRP
EMKPVDVGRP VIRPPEQNAP PPGAPDKDKQ KQEPKTPVAP KKDLKIKNMG
SWASLVQKHP TTPSSTAKSS SDSFEQFRRA AREKEEREKA LKAQAEHAEK
EKERLRQERM RSREDEDALE QARRAHEEAR RRQEQQQQQR QEQQQQQQQQ
AAAVAAAATP QAQSSQPQSM LDQQRELARK REQERRRREA MAATIDMNFQ
SDLLSIFEEN LF
UCSC uc002nar.4; human. [O60885-1];

Category

Human BRD4 ELISA Kit

Human Protein HUNK1 ELISA Kit;Human HUNK1 ELISA Kit;Human CAP ELISA Kit;Human HUNKI ELISA Kit;Human MCAP ELISA Kit;Human bromodomain containing 4 ELISA Kit;Human bromodomain-containing protein 4 ELISA Kit;Human chromosome-associated protein ELISA Kit;