Human BRD3 ELISA Kit

Alternative name
Human RING3-like protein ELISA Kit;Human KIAA0043 ELISA Kit;Human RING3L ELISA Kit;Human ORFX ELISA Kit;Human bromodomain containing 3 ELISA Kit;Human bromodomain-containing protein 3 ELISA Kit;Human bromodomain-containing protein 3 short isoform ELISA Kit;
Catalog
E005339
species
Human
GeneBRD3
Other Species Mouse Brd3 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of human BRD3. No significant cross-reactivity or interference between human BRD3 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human BRD3 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseHuman BRD3 ELISA Kit allows for the in vitro quantitative determination of BRD3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BRD3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BRD3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BRD3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BRD3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Bromodomain-containing protein 3 Protein information
Uniprot ID BRD3_HUMAN
Uniprot AC Q15059; B1APD9; Q4G5Y3; Q5T1R7; Q8N5M3; Q92645;
UniGene Hs.522472; Hs.654869;
GeneID 8019
KEGG hsa:8019;
Human Bromodomain-containing protein 3 Protein SEQUENCE
SEQUENCE 726 AA; 79542 MW; 64F526FC3C1033AA CRC64;
MSTATTVAPA GIPATPGPVN PPPPEVSNPS KPGRKTNQLQ YMQNVVVKTL
WKHQFAWPFY QPVDAIKLNL PDYHKIIKNP MDMGTIKKRL ENNYYWSASE
CMQDFNTMFT NCYIYNKPTD DIVLMAQALE KIFLQKVAQM PQEEVELLPP
APKGKGRKPA AGAQSAGTQQ VAAVSSVSPA TPFQSVPPTV SQTPVIAATP
VPTITANVTS VPVPPAAAPP PPATPIVPVV PPTPPVVKKK GVKRKADTTT
PTTSAITASR SESPPPLSDP KQAKVVARRE SGGRPIKPPK KDLEDGEVPQ
HAGKKGKLSE HLRYCDSILR EMLSKKHAAY AWPFYKPVDA EALELHDYHD
IIKHPMDLST VKRKMDGREY PDAQGFAADV RLMFSNCYKY NPPDHEVVAM
ARKLQDVFEM RFAKMPDEPV EAPALPAPAA PMVSKGAESS RSSEESSSDS
GSSDSEEERA TRLAELQEQL KAVHEQLAAL SQAPVNKPKK KKEKKEKEKK
KKDKEKEKEK HKVKAEEEKK AKVAPPAKQA QQKKAPAKKA NSTTTAGRQL
KKGGKQASAS YDSEEEEEGL PMSYDEKRQL SLDINRLPGE KLGRVVHIIQ
SREPSLRDSN PDEIEIDFET LKPTTLRELE RYVKSCLQKK QRKPFSASGK
KQAAKSKEEL AQEKKKELEK RLQDVSGQLS SSKKPARKEK PGSAPSGGPS
RLSSSSSSES GSSSSSGSSS DSSDSE
UCSC uc004cew.4; human. [Q15059-1];

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anti-BRD3 antibody

Category

Human BRD3 ELISA Kit

Human RING3-like protein ELISA Kit;Human KIAA0043 ELISA Kit;Human RING3L ELISA Kit;Human ORFX ELISA Kit;Human bromodomain containing 3 ELISA Kit;Human bromodomain-containing protein 3 ELISA Kit;Human bromodomain-containing protein 3 short isoform ELISA Kit;