Human BRF2 ELISA Kit

Alternative name
Human TFIIIB50 ELISA Kit;Human hTFIIIB50 ELISA Kit;Human B-related factor 2 ELISA Kit;Human BRF-2 ELISA Kit;Human hBRFU ELISA Kit;Human BRFU ELISA Kit;Human BRF2, RNA polymerase III transcription initiation factor subunit ELISA Kit;Human transcription factor IIIB 50 kDa subunit ELISA Kit;Human BRF2, RNA polymerase III transcription initiation factor 50 kDa subunit ELISA Kit;Human BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like ELISA Kit;Human RNA polymerase III transcription initiation factor BRFU ELISA Kit;Human transcription factor IIB- related factor, TFIIIB50 ELISA Kit;
Catalog
E005313
species
Human
GeneBRF2
Standard Curve
Other Species Mouse Brf2 ELISA Kit Bovine BRF2 ELISA Kit Monkey BRF2 ELISA Kit Rat Brf2 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of human BRF2. No significant cross-reactivity or interference between human BRF2 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human BRF2 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman BRF2 ELISA Kit allows for the in vitro quantitative determination of BRF2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BRF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BRF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BRF2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BRF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Transcription factor IIIB 50 kDa subunit Protein information
Uniprot ID BRF2_HUMAN
Uniprot AC Q9HAW0; B2RD62; B4DFZ6; D3DSW6; Q9H2Y3; Q9H3B3; Q9NUY6;
UniGene Hs.709301;
GeneID 55290
KEGG hsa:55290;
Human Transcription factor IIIB 50 kDa subunit Protein SEQUENCE
SEQUENCE 419 AA; 46533 MW; 90A39F72DBA14888 CRC64;
MPGRGRCPDC GSTELVEDSH YSQSQLVCSD CGCVVTEGVL TTTFSDEGNL
REVTYSRSTG ENEQVSRSQQ RGLRRVRDLC RVLQLPPTFE DTAVAYYQQA
YRHSGIRAAR LQKKEVLVGC CVLITCRQHN WPLTMGAICT LLYADLDVFS
STYMQIVKLL GLDVPSLCLA ELVKTYCSSF KLFQASPSVP AKYVEDKEKM
LSRTMQLVEL ANETWLVTGR HPLPVITAAT FLAWQSLQPA DRLSCSLARF
CKLANVDLPY PASSRLQELL AVLLRMAEQL AWLRVLRLDK RSVVKHIGDL
LQHRQSLVRS AFRDGTAEVE TREKEPPGWG QGQGEGEVGN NSLGLPQGKR
PASPALLLPP CMLKSPKRIC PVPPVSTVTG DENISDSEIE QYLRTPQEVR
DFQRAQAARQ AATSVPNPP
UCSC uc003xkk.4; human. [Q9HAW0-1];

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Human BRF2 ELISA Kit