Mouse Fgf23 ELISA Kit

Alternative name
Mouse FGF-23 ELISA Kit;Mouse fibroblast growth factor 23 ELISA Kit;
Catalog
E052898
species
Mouse
GeneFgf23
SpecificityThis kit recognizes natural and recombinant Mouse FGF23. No significant cross-reactivity or interference between Mouse FGF23 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity9.375pg/mL
Intended UseMouse Fgf23 ELISA Kit allows for the in vitro quantitative determination of Fgf23 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse FGF23 concentrations in serum, plasma and other biological fluids. Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FGF23. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FGF23 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FGF23, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of FGF23. You can calculate the concentration of FGF23 in the samples by comparing the OD of the samples to the standard curve.
Mouse Fibroblast growth factor 23 Protein information
Uniprot ID FGF23_MOUSE
Uniprot AC Q9EPC2;
UniGene Mm.347933;
GeneID 64654
KEGG mmu:64654;
Mouse Fibroblast growth factor 23 Protein SEQUENCE
SEQUENCE 251 AA; 27758 MW; 110C1F2C735DC360 CRC64;
MLGTCLRLLV GVLCTVCSLG TARAYPDTSP LLGSNWGSLT HLYTATARTS
YHLQIHRDGH VDGTPHQTIY SALMITSEDA GSVVITGAMT RRFLCMDLHG
NIFGSLHFSP ENCKFRQWTL ENGYDVYLSQ KHHYLVSLGR AKRIFQPGTN
PPPFSQFLAR RNEVPLLHFY TVRPRRHTRS AEDPPERDPL NVLKPRPRAT
PVPVSCSREL PSAEEGGPAA SDPLGVLRRG RGDARGGAGG ADRCRPFPRF
V
UCSC uc009dvp.2; mouse.;

Category

Mouse Fgf23 ELISA Kit

Mouse FGF-23 ELISA Kit;Mouse fibroblast growth factor 23 ELISA Kit;