Mouse FTL ELISA Kit

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  • Alternative name

    Ferritin light chain ELISA KIT; FTL ELISA KIT; Ferritin L subunit ELISA KIT

  • Catalog
    E052873
  • species
    Mouse
  • GeneFTL
  • Standard CurveMouse FTL ELISA Kit
  • Other Species Human FTL ELISA KitBovine FTL ELISA KitCanine FTL ELISA KitHorse FTL ELISA KitPorcine FTL ELISA KitRabbit FTL ELISA KitSheep FTL ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Ferritin, Light Polypeptide (FTL). No significant cross-reactivity or interference between Ferritin, Light Polypeptide (FTL) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.53ng/mL.
  • Intended UseMouse FTL ELISA Kit allows for the in vitro quantitative determination of FTL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ferritin, Light Polypeptide (FTL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ferritin, Light Polypeptide (FTL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ferritin, Light Polypeptide (FTL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ferritin, Light Polypeptide (FTL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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