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Alternative name
Bile acyl-CoA synthetase ELISA KIT; Bile acid-CoA ligase ELISA KIT; BA-CoA ligase ELISA KIT; BAL ELISA KIT; Cholate--CoA ligase ELISA KIT; Fatty acid transport protein 5 ELISA KIT; FATP-5 ELISA KIT; Solute carrier family 27 member 5 ELISA KIT; Very long-chain acyl-CoA synthetase-related protein ELISA KIT; VLACS-related ELISA KIT; VLACSRSlc27a5 ELISA KIT; Acsb ELISA KIT; Acsvl6 ELISA KIT; Fatp5 ELISA KIT; Vlacsr ELISA KIT; BACS ELISA KIT; BA-CoA ligase ELISA KIT; BAL ELISA KIT; FATP-5 ELISA KIT; VLACS-related ELISA KIT; VLACSR ELISA KIT
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Catalog
E052774
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species
Mouse
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GeneFATP5
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SpecificityThis assay has high sensitivity and excellent specificity for detection of FATP5. No significant cross-reactivity or interference between FATP5 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of mouse FATP5 is typically less than 0.054ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by add
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Intended UseMouse FATP5 ELISA Kit allows for the in vitro quantitative determination of FATP5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FATP5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FATP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FATP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FATP5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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