Mouse FXR ELISA Kit

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  • Alternative name

    Bile acid receptor ELISA KIT; Farnesoid X-activated receptor ELISA KIT; Farnesol receptor HRR-1 ELISA KIT; Nuclear receptor subfamily 1 group H member 4 ELISA KIT; Retinoid X receptor-interacting protein 14 ELISA KIT; RXR-interacting protein 14NR1H4 ELISA KIT; BAR ELISA KIT; FXR ELISA KIT; HRR1 ELISA KIT; RIP14 ELISA KIT; RXR-interacting protein 14 ELISA KIT

  • Catalog
    E052684
  • species
    Mouse
  • GeneFXR
  • Standard CurveMouse FXR ELISA Kit
  • Other Species Human FXR ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Farnesoid X Receptor (FXR). No significant cross-reactivity or interference between Farnesoid X Receptor (FXR) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.051ng/mL.
  • Intended UseMouse FXR ELISA Kit allows for the in vitro quantitative determination of FXR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Farnesoid X Receptor (FXR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Farnesoid X Receptor (FXR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Farnesoid X Receptor (FXR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Farnesoid X Receptor (FXR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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