Mouse Fads3 ELISA Kit

Alternative name
Mouse AI464531 ELISA Kit;Mouse fatty acid desaturase 3 ELISA Kit;
Catalog
E052475
species
Mouse
GeneFads3
SpecificityThis assay has high sensitivity and excellent specificity for detection of Fads3 . No significant cross-reactivity or interference between Fads3 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 0.094ng/ml
Intended UseMouse Fads3 ELISA Kit allows for the in vitro quantitative determination of Fads3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Fads3antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Fads3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Fads3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Fads3 can be calculated.
Mouse Fatty acid desaturase 3 Protein information
Uniprot ID FADS3_MOUSE
Uniprot AC Q9JJE7; Q7TND7; Q8C4Y5; Q8CDZ4;
UniGene Mm.253875;
GeneID 60527
KEGG mmu:60527;
Mouse Fatty acid desaturase 3 Protein SEQUENCE
SEQUENCE 449 AA; 51469 MW; 0E0B74EC8C9E5199 CRC64;
MGGVGEPGGG PGPREGPAPL GAPLPIFRWE QIRQHDLPGD KWLVIERRVY
DISRWAQRHP GGSRLIGHHG AEDATDAFHA FHQDLHFVRK FLKPLLIGEL
APEEPSQDGA QNAQLIEDFR ALRQAAEDMK LFEADTTFFA LLLGHILAME
LLAWLIIYLL GPGWVSSILA ALILAISQAQ CWCLQHDLGH ASIFTKSRWN
HVAQQFVMGQ LKGFSAHWWN FRHFQHHAKP NIFHKDPDVT VAPVFLLGES
SVEYGKKKRR YLPYNHQHLY FFLIGPPLLT LVNFEVENLA YMLVCMQWTD
LLWAASFYSR FFLSYSPFYG ATGTLLLFVA VRVLESHWFV WITQMNHIPK
EIGHEKHRDW ASSQLAATCN VEPSLFIDWF SGHLNFQIEH HLFPTMPRHN
YRRVAPLVKA FCAKHGLHYE VKPFLTALVD IIGSLKKSGD IWLDAYLHQ
UCSC uc008goz.1; mouse.;

Category

Mouse Fads3 ELISA Kit

Mouse AI464531 ELISA Kit;Mouse fatty acid desaturase 3 ELISA Kit;