Mouse Fads2 ELISA Kit

Alternative name
Mouse delta(6) fatty acid desaturase ELISA Kit;Mouse D6D ELISA Kit;Mouse delta(6) desaturase ELISA Kit;Mouse Delta-6 desaturase ELISA Kit;Mouse Fadsd2 ELISA Kit;Mouse 2900042M13Rik ELISA Kit;Mouse fatty acid desaturase 2 ELISA Kit;
Catalog
E052474
species
Mouse
GeneFads2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Fads2 . No significant cross-reactivity or interference between Fads2 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 0.094ng/ml
Intended UseMouse Fads2 ELISA Kit allows for the in vitro quantitative determination of Fads2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Fads2antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Fads2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Fads2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Fads2 can be calculated.
Mouse Fatty acid desaturase 2 Protein information
Uniprot ID FADS2_MOUSE
Uniprot AC Q9Z0R9;
UniGene Mm.38901;
GeneID 56473
KEGG mmu:56473;
Mouse Fatty acid desaturase 2 Protein SEQUENCE
SEQUENCE 444 AA; 52387 MW; 7615D17024D3B771 CRC64;
MGKGGNQGEG STERQAPMPT FRWEEIQKHN LRTDRWLVID RKVYNVTKWS
QRHPGGHRVI GHYSGEDATD AFRAFHLDLD FVGKFLKPLL IGELAPEEPS
LDRGKSSQIT EDFRALKKTA EDMNLFKTNH LFFFLLLSHI IVMESLAWFI
LSYFGTGWIP TLVTAFVLAT SQAQAGWLQH DYGHLSVYKK SIWNHVVHKF
VIGHLKGASA NWWNHRHFQH HAKPNIFHKD PDIKSLHVFV LGEWQPLEYG
KKKLKYLPYN HQHEYFFLIG PPLLIPMYFQ YQIIMTMISR RDWVDLAWAI
SYYMRFFYTY IPFYGILGAL VFLNFIRFLE SHWFVWVTQM NHLVMEIDLD
HYRDWFSSQL AATCNVEQSF FNDWFSGHLN FQIEHHLFPT MPRHNLHKIA
PLVKSLCAKH GIEYQEKPLL RALIDIVSSL KKSGELWLDA YLHK
UCSC uc008gpb.1; mouse.;

Category

Mouse Fads2 ELISA Kit

Mouse delta(6) fatty acid desaturase ELISA Kit;Mouse D6D ELISA Kit;Mouse delta(6) desaturase ELISA Kit;Mouse Delta-6 desaturase ELISA Kit;Mouse Fadsd2 ELISA Kit;Mouse 2900042M13Rik ELISA Kit;Mouse fatty acid desaturase 2 ELISA Kit;