Mouse Akr1c6 ELISA Kit

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  • Alternative name

    Mouse 17-beta-HSD 5 ELISA Kit;Mouse Hsd17b5 ELISA Kit;Mouse 3alpha-HSD ELISA Kit;Mouse Akr1c1 ELISA Kit;Mouse aldo-keto reductase family 1, member C6 ELISA Kit;Mouse estradiol 17 beta-dehydrogenase 5 ELISA Kit;Mouse 17HSD type 5 ELISA Kit;Mouse aldo-keto reductase family 1, member C1 (aldehyde reductase) ELISA Kit;Mouse estradiol 17-beta-dehydrogenase (A-specific) ELISA Kit;Mouse hydroxysteroid 17-beta dehydrogenase 5 ELISA Kit;

  • Catalog
    E052137
  • species
    Mouse
  • GeneAkr1c6
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseMouse Akr1c6 ELISA Kit allows for the in vitro quantitative determination of Akr1c6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Akr1c6 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Akr1c6 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Akr1c6 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Akr1c6 can be calculated.
  • Mouse Estradiol 17 beta-dehydrogenase 5 Protein information
  • Uniprot ID DHB5_MOUSE
  • Uniprot AC P70694;
  • UniGene Mm.196666;
  • GeneID 83702
  • KEGG mmu:83702;
  • Mouse Estradiol 17 beta-dehydrogenase 5 Protein SEQUENCE
  • SEQUENCE 323 AA; 37048 MW; 7A1B195E3074D36D CRC64;

    MDSKQQTVRL SDGHFIPILG FGTYAPQEVP KSKATEATKI AIDAGFRHID

    SASMYQNEKE VGLAIRSKIA DGTVKREDIF YTSKVWCTFH RPELVRVCLE

    QSLKQLQLDY VDLYLIHFPM AMKPGENYLP KDENGKLIYD AVDICDTWEA

    MEKCKDAGLA KSIGVSNFNR RQLEKILKKP GLKYKPVCNQ VECHPYLNQG

    KLLDFCRSKD IVLVAYSALG SHREKQWVDQ SSPVLLDNPV LGSMAKKYNR

    TPALIALRYQ LQRGVVVLAK SFSEKRIKEN MQVFEFQLTS EDMKVLDDLN

    KNIRYISGSS FKDHPDFPFW DEY

  • UCSC uc007pjo.1; mouse.;




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