Intended UseHuman ACVA ELISA Kit allows for the in vitro quantitative determination of ACVA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of ACVA in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay||This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to ACVA has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled ACVA and unlabeled ACVA (Standards or samples) with the pre-coated antibody specific to ACVA. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of ACVA in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of ACVA in the sample.