Mouse Elane ELISA Kit

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  • Alternative name

    Mouse Elastase-2 ELISA Kit;Mouse leukocyte elastase ELISA Kit;Mouse Ela2 ELISA Kit;Mouse F430011M15Rik ELISA Kit;Mouse NE ELISA Kit;Mouse elastase, neutrophil expressed ELISA Kit;Mouse neutrophil elastase ELISA Kit;Mouse elastase 2, neutrophil ELISA Kit;

  • Catalog
    E051649
  • species
    Mouse
  • GeneElane
  • Standard CurveMouse Elane ELISA Kit
  • Other Species Human ELANE ELISA Kit
  • SpecificityNatural and recombinant mouse Elastase
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of mouse Elastase in cell culture supernates, serum and p
  • Intended UseMouse Elane ELISA Kit allows for the in vitro quantitative determination of Elane , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: Mouse Elastase ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Elastase has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: S27-R260) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Elastase is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Elastase amount of sample captured in plate. Background: Neutrophil elastase is a serine protease of neutrophil and monocyte granules. Its key physiologic role is in innate host defense, but it can also participate in tissue remodeling and possesses secretagogue actions important to local inflammatory responses. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. This protein also degrades the outer membrane protein A (OmpA) of E Coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.
  • Mouse Neutrophil elastase Protein information
  • Uniprot ID ELNE_MOUSE
  • Uniprot AC Q3UP87; A6H698; Q61515;
  • UniGene Mm.262194;
  • GeneID 50701
  • KEGG mmu:50701;
  • Mouse Neutrophil elastase Protein SEQUENCE
  • SEQUENCE 265 AA; 28648 MW; 666029A299275EB6 CRC64;

    MALGRLSSRT LAAMLLALFL GGPALASEIV GGRPARPHAW PFMASLQRRG

    GHFCGATLIA RNFVMSAAHC VNGLNFRSVQ VVLGAHDLRR QERTRQTFSV

    QRIFENGFDP SQLLNDIVII QLNGSATINA NVQVAQLPAQ GQGVGDRTPC

    LAMGWGRLGT NRPSPSVLQE LNVTVVTNMC RRRVNVCTLV PRRQAGICFG

    DSGGPLVCNN LVQGIDSFIR GGCGSGLYPD AFAPVAEFAD WINSIIRSHN

    DHLLTHPKDR EGRTN

  • UCSC uc007gai.1; mouse.;



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