Mouse ELA2 ELISA Kit

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  • Alternative name

    Neutrophil elastase ELISA KIT; Bone marrow serine protease ELISA KIT; Elastase-2 ELISA KIT; Human leukocyte elastase ELISA KIT; HLE ELISA KIT; Medullasin ELISA KIT; PMN elastaseELANE ELISA KIT; ELA2 ELISA KIT; HLE ELISA KIT

  • Catalog
    E051636
  • species
    Mouse
  • GeneELA2
  • Other Species Human ELA2 ELISA KitHuman NE/ELA2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse NE. No significant cross-reactivity or interference between mouse NE and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of mouse NE is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseMouse ELA2 ELISA Kit allows for the in vitro quantitative determination of ELA2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NE bound in the initial step. The color development is stopped and the intensity of the color is measured.


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