Mouse Huwe1 ELISA Kit

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  • Alternative name

    Mouse E3Histone ELISA Kit;Mouse HECT, UBA and WWE domain-containing protein 1 ELISA Kit;Mouse HECT-type E3 ubiquitin transferase HUWE1 ELISA Kit;Mouse Upstream regulatory element-binding protein 1 ELISA Kit;Mouse URE-B1 ELISA Kit;Mouse URE-binding protein 1 ELISA Kit;Mouse Kiaa0312 ELISA Kit;Mouse Ureb1 ELISA Kit;Mouse 5430439H10Rik ELISA Kit;Mouse AU041296 ELISA Kit;Mouse Arf-bp1 ELISA Kit;Mouse C430014N20Rik ELISA Kit;Mouse C80292 ELISA Kit;Mouse Gm1718 ELISA Kit;Mouse Ib772 ELISA Kit;Mouse LASU1 ELISA Kit;Mouse Mule ELISA Kit;Mouse HECT, UBA and WWE domain containing 1 ELISA Kit;Mouse E3 ubiquitin-protein ligase HUWE1 ELISA Kit;Mouse Mcl-1 ubiquitin ligase ELISA Kit;Mouse upstream regulatory element binding protein 1 ELISA Kit;

  • Catalog
    E051468
  • species
    Mouse
  • GeneHuwe1
  • Other Species Human HUWE1 ELISA KitRat Huwe1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.188ng/ml
  • Intended UseMouse Huwe1 ELISA Kit allows for the in vitro quantitative determination of Huwe1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Huwe1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Huwe1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Huwe1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Huwe1 can be calculated.
  • Mouse E3 ubiquitin-protein ligase HUWE1 Protein information
  • Uniprot ID HUWE1_MOUSE
  • Uniprot AC Q921M5; Q9R0P2;
  • UniGene Mm.27372; Mm.488413;
  • GeneID 59026
  • KEGG mmu:59026;
  • Mouse E3 ubiquitin-protein ligase HUWE1 Protein SEQUENCE
  • SEQUENCE 4377 AA; 482635 MW; 22700C859CD9AF83 CRC64;

    MKVDRTKLKK TPTEAPADCR ALIDKLKVCN DEQLLLELQQ IKTWNIGKCE

    LYHWVDLLDR FDGILADAGQ TVENMSWMLV CDRPEKEQLK MLLLAVLNFT

    ALLIEYSFSR HLYSSIEHLT TLLASSDMQV VLAVLNLLYV FSKRSNYITR

    LGSDKRTPLL TRLQHLAESW GGKENGFGLA ECCRDLQMLK YPPSATTLHF

    EFYADPGAEV KIEKRTTSNT LHYIHIEQLD KISESPSEIM ESLTKMYSIP

    KDKQMLLFTH IRLAHGFSNH RKRLQAVQAR LHAISILVYS NALQESANSI

    LYNGLIEELV DVLQITDKQL MEIKAASLRT LTSIVHLERT PKLSSIIDCT

    GTASYHGFLP VLVRNCIQAM IDPSMDPYPH QFATALFSFL YHLASYDAGG

    EALVSCGMME ALLKVIKFLG DEQDQITFVT RAVRVVDLIT NLDMAAFQSH

    SGLSIFIYRL EHEVDLCRKE CPFVIKPKIQ RPSTTQEGEE METDMDGVQC

    IPQRAALLKS MLNFLKKAIQ DPAFSDGIRH VMDGSLPTSL KHIISNAEYY

    GPSLFLLATE VVTVFVFQEP SLLSSLQDNG LTDVMLHALL IKDVPATREV

    LGSLPNVFSA LCLNARGLQS FVQCQPFERL FKVLLSPDYL PAMRRRRSSD

    PLGDTASNLG SAVDELMRHQ PTLKTDATTA IIKLLEEICN LGRDPKYICQ

    KPSIQKADGT ATAPPPRSNH AAEEASSEDE EEEEVQAMQS FNSAQQNETE

    PNQQVVGTEE RIPIPLMDYI LNVMKFVESI LSNNTTDDHC QEFVNQKGLL

    PLVTILGLPN LPIDFPTSAA CQAVAGVCKS ILTLSHEPKV LQEGLLQLDL

    ILSSLEPLHR PIESPGGSVL LRELACAGNV ADATLSAQAT PLLHALTAAH

    AYIMMFVHTC RVGQSEIRSI SVNQWGSQLG LSVLSKLSQL YCSLVWESTV

    LLSLCTPNSL PSGCEFGQAD MQKLVPKDEK AGTTQGGKRS DGEQDGTAGS

    MDASAQGLLE GIELDGDTLA PMETDEPSSS DSKGKSKITP AMAARIKQIK

    PLLSASSRLG RALAELFGLL VKLCVGSPVR QRRSHHAAST TTAPTPAARS

    TASALTKLLT KGLSWQPPPY TPTPRFRLTF FICSVGFTSP MLFDERKYPY

    HLMLQKFLCS GGHNALFETF NWALSMGGKV PVSEGLEHSD LPDGTGEFLD

    AWLMLVEKMV NPTTVLESPH SLPAKLPGGV QSFPQFSALR FLVVTQKAAF

    TCIKNLWNRK PLKVYGGRMA ESMLAILCHI LRGEPVIRER LSKEKEGSRG

    EEEAGQEEGG SRREPQVNQQ QLQQLMDMGF TREHAMEALL NTSTMEQATE

    YLLTHPPPII GGVVRDLSMS EEDQMMRAIA MSLGQDIPMD QRAESPEEVA

    CRKEEEERKA REKQEEEEAK CLEKFQDADP LEQDELHTFT DTMLPGCFHL

    LDELPDTVYR VCDLIMTAIK RNGADYRDMI LKQVVNQVWE AADVLIKAAL

    PLTTSDTKTV SEWISQMATL PQASNLATRI LLLTLLFEEL KLPCAWVVES

    SGILNVLIKL LEVVQPCLQA AKEQKEVQTP KWITPVLLLI DFYEKTAISS

    KRRAQMTKYL QSNSNNWRWF DDRSGRWCSY SASNNSTIDS AWKSGETSVR

    FTAGRRRYTV QFTTMVQVNE ETGNRRPVML TLLRVPRLSK NSKSSNGQEL

    EKTLEESKET DIKHKENKGN DIPLALESTN TEKEASLDET KIGEILIQGL

    TEDMVTVLIR ACVSMLGVPV DPDTLHATLR LCLRLTRDHK YAMMFAELKS

    TRMILNLTQS SGFNGFTPLV TLLLRHIIED PCTLRHTMEK VVRSAATSGA

    GSTTSGVVSG SLGSREINYI LRVLGPAACR NPDIFTEVAN CCIRIALPAP

    RGSGTASDDE FENLRIKGPN AVQLVKTTPL KPSSLPVIPD TIKEVIYDML

    NALAAYHAPE EADKSDPKPG GTTQEVGQLL QDMGDDVYQQ YRSLTRQSSD

    FDTQSGFSLN SQVFAADGAP AETSTTGTSQ GEASTPEETR EGKKDKEGDR

    TSEEGKQKSK GSKPLMPTST ILRLLAELVR SYVGIATLIA NYSYTVGQSE

    LIKEDCSVLA FVLDHLLPHT QNAEDKDTPA LARLFLASLA AAGSGTDAQV

    ALVNEVKAAL GRALAMAEST EKHARLQAVM CIISTIMESC PSTSSFYSSA

    TAKTQHNGMN NIIRLFLKKG LVNDLARVPH SLDLSSPNMA NTVNAALKPL

    ETLSRIVNQP SSLFGSKSAS SKNKSEQDAQ GASQDSSSHQ QDPGEPGEAE

    VQEEDHDVTQ TEVADGDIMD GEAETDSVVI AGQPEVLSSQ EMQVENELED

    LIDELLERDG GSGNSTIIVS RSGEDESQED VLMDEAPSNL SQASTLQANR

    EDSMNILDPE DEEEHTQEED SSGSNEDEDD SQDEEEEEEE DEEDDQEDDE

    GEEGDEDDDD DGSEMELDED YPDMNASPLV RFERFDREDD LIIEFDNMFS

    SATDIPPSPG NIPTTHPLMV RHADHSSLTL GSGSSTTRLT QGIGRSQRTL

    RQLTANTGHT IHVHYPGNRQ PNPPLILQRL LGPSAAADIL QLSSSLPLQS

    RGRARLLVGN DDVHIIARSD DELLDDFFHD QSTATSQAGT LSSIPTALTR

    WTEECKVLDA ESMHDCVSVV KVPIVNHLEF LRDEELEERR EKRRKQLAEE

    ETKIIDKGKE DKENRDQSAQ CTVTKTNDST EQNVSDGTPM PDSYPTTPSS

    TDAPTSESKE TLGTLQPSQQ QPALPPPPSL GEIPQELQSP AEEVANSTQL

    LMPIELEELG PTRPSGEAET TQMELSPAPT ITSLSPERAE DSDALTAVSS

    QLEGSPMDTS SLASCTLEEA VGDTPAAGSS EQPTAGSSTP GDAPSVVAEV

    QGRPDVSRES NQPPEDSSPP ASSESSSTRD SAVAISGADS RGILEEPLPS

    TSSEEEDPLA GISLPEGVDP SFLAALPDDI RREVLQNQLG IRPPTRSAPS

    SNSSAPAVVG NPGVTEVSPE FLAALPPAIQ EEVLAQQRAE QQRRELAQNA

    SSDTPMDPVT FIQTLPSDLR RSVLEDMEDS VLAVMPPDIA AEAQALRREQ

    EARQRQLMHE RLFGHSSTSA LSAILRSPAF TSRLSGNRGV QYTRLAVQRG

    GTFQMGGSSS HNRPSGSNVD TLLRLRGRLL LDHEALSCLL VLLFVDEPKL

    NTSRLHRVLR NLCYHAQTRH WVIRSLLSIL QRSSESELCI ETPKLSTSEE

    RGKKSSKSCA SSSHENRPLD LLHKMESKSS NQLSWLSVSM DAALGCRTNI

    FQIQRSGGRK HTEKHASSGS TVHIHPQAAP VVCRHVLDTL IQLAKVFPSH

    FTQQRTKETN CESDRERGSK QACSPCSSQS SSSGICTDFW DLLVKLDNMN

    VSRKGKNSVK SVPVSSGGEG ETSPHSLEAS PLGQLMNMLS HPVIRRSSLL

    TEKLLRLLSL ISIALPENKV SEVQTNSSNS GSSTAATSNT STTTTTTTTA

    TAPTPTPPAA TTPVTSAPAL VAATAISTIT VAASTTVTTP TTATTTVSTS

    TTKGSKSPAK VGEGGSGIDF KMVSSGLTEN QLQLSVEVLT SHSCSEEGLE

    DAANVLLQLS RGDSGTRDTV LKLLLNGARH LGYTLCKQIG TLLAELREYN

    LEQQRRAQCE TLSPDGLPEE QPQTTKLKGK MQSRFDMAEN VVIVASQKRP

    LGGRELQLPS MSMLTSKTST QKFFLRVLQV IIQLRDDTRR ANKKAKQTGR

    LGSSGLGSAS SIQAAVRQLE AEADAIIQMV REGQRARRQQ QAATSESSNQ

    SETSVRREES PMDVDQPSPS AQDTQSIVIS DGTPQGEKEK EEKPPELPLL

    SEQLSLDELW DMLGECLKEL EESHDQHAVL VLQPAVEAFF LVHATERESK

    PPVRDTRESQ LAHIKDEPPP LSPAPLTPAT PSSLDPFFSR EPSSMHISSS

    LPPDTQKFLR FAETHRTVLN QILRQSTTHL ADGPFAVLVD YIRVLDFDVK

    RKYFRQELER LDEGLRKEDM AVHVRRDHVF EDSYRELHRK SPEEMKNRLY

    IVFEGEEGQD AGGLLREWYM IISREMFNPM YALFRTSPGD RVTYTINPSS

    HCNPNHLSYF KFVGRIVAKA VYDNRLLECY FTRSFYKHIL GKSVRYTDME

    SEDYHFYQGL VYLLENDVST LGYDLTFSTE VQEFGVCEVR DLKPNGANIL

    VTEENKKEYV HLVCQMRMTG AIRKQLAAFL EGFYEIIPKR LISIFTEQEL

    ELLISGLPTI DIDDLKSNTE YHKYQSNSIQ IQWFWRALRS FDQADRAKFL

    QFVTGTSKVP LQGFAALEGM NGIQKFQIHR DDRSTDRLPS AHTCFNQLDL

    PAYESFEKLR HMLLLAIQEC SEGFGLA

  • UCSC uc009upr.1; mouse. [Q7TMY8-2];


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