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Mouse Hace1 ELISA Kit

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  • Alternative name

    Mouse HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1 ELISA Kit;Mouse HECT-type E3 ubiquitin transferase HACE1 ELISA Kit;Mouse Kiaa1320 ELISA Kit;Mouse 1700042J16Rik ELISA Kit;Mouse A730034A22Rik ELISA Kit;Mouse BC025474 ELISA Kit;Mouse HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 ELISA Kit;Mouse E3 ubiquitin-protein ligase HACE1 ELISA Kit;
  • Catalog
    E051467
  • species
    Mouse
  • GeneHace1
  • SpecificityThis assay recognizes recombinant and natural mouse E3 ubiquitin-protein ligase HACE1. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Hace1 ELISA Kit allows for the in vitro quantitative determination of Hace1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to E3 ubiquitin-protein ligase HACE1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for E3 ubiquitin-protein ligase HACE1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain E3 ubiquitin-protein ligase HACE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of E3 ubiquitin-protein ligase HACE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse E3 ubiquitin-protein ligase HACE1 Protein information
  • Uniprot ID HACE1_MOUSE
  • Uniprot AC Q3U0D9; F6VQI5; F7ALT5; Q5DTY7; Q8BXY2; Q8R160; Q8R3G4;
  • UniGene Mm.458633;
  • GeneID 209462
  • KEGG mmu:209462;
  • Mouse E3 ubiquitin-protein ligase HACE1 Protein SEQUENCE

  • SEQUENCE 909 AA; 102114 MW; 804F62A06E9C78A0 CRC64;

    MERAMEQLNR LTRSLRRART VELPEDNETA VYTLMPMVMA DQHRSVSELL

    SNSKFDVNYA FGRVKRSLLH IAANCGSVEC LVLLLKKGAN PNYQDISGCT

    PLHLAARNGQ KKCMSKLLEY SADVNICNNE GLTAIHWLAV NGRTELLHDL

    VQHVTDVDVE DAMGQTALHV ACQNGHKTTV QCLLDSGADI NRPNVSGATP

    LYFACSHGQR DTAQILLLRG AKYLPDKNGV TPLDLCVQGG YGQTCEVLIQ

    YHPRLFQTIV QMTQNEDLRE NMLRQVLQHL SQQSESQYLK ILTGLAEVAT

    TNGHKLLSLS SNYDAQMKSL LRIVRIFCHV FRIGPSSPSN GIDMGYNGNK

    TPRSQVFKPL ELLWHSLDEW LVLIATELMK NKEDSTDITS ILLKQKGQDQ

    EAPSLSAFEP PGPGSYESLP PGPGDSKPEV LAGEQEASAD CQDVISVTAN

    RLSAVIQAFY MCCSCQMPPG MTSPRFIEFV CKHDEVLKCF VNRNPKIIFD

    HFHFLLECPE LMSRFMHIIK AQPFKDRCEW FYEHLHSGQP DSDMVHRPVS

    ENDILLVHRD SIFRSSCEIV SKANCAKLKQ GIAVRFHGEE GMGQGVVREW

    FDILSNEIVN PDYALFTQSA DGTTFQPNSN SYVNPDHLNY FRFAGQILGL

    ALNHRQLVNI YFTRSFYKHI LGIPVNYQDV ASIDPEYAKN LQWILDNDIS

    DLGLELTFSV ETDVFGAMEE VPLKPGGGSI LVTQNNKAEY VQLVTELRMT

    RAIQPQINAF LQGFHMFIPP SLIQLFDEYE LELLLSGMPE IDVNDWIKNT

    EYTSGYERED PVIQWFWEVV EDITQEERVL LLQFVTGSSR VPHGGFANIM

    GGSGLQNFTI AAVPYTPNLL PTSSTCINML KLPEYPSKEI LKDRLLVALH

    CGSYGYTMA

  • UCSC uc007fad.2; mouse. [Q3U0D9-1];



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