Mouse DPD ELISA Kit

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  • Alternative name

    Dihydropyrimidine dehydrogenase [NADP(+)] ELISA KIT; Dihydrothymine dehydrogenase ELISA KIT; Dihydrouracil dehydrogenaseDpyd ELISA KIT; DPD ELISA KIT; DHPDHase ELISA KIT; DPD ELISA KIT

  • Catalog
    E051273
  • species
    Mouse
  • GeneDPD
  • Other Species Human DPD ELISA KitHuman DPD ELISA KitBovine DPD ELISA KitCanine DPD ELISA KitChicken DPD ELISA KitGeneral DPD ELISA KitPorcine DPD ELISA KitRabbit DPD ELISA KitRat DPD ELISA KitCamel DPD ELISA KitGoat DPD ELISA KitGuinea Pig DPD ELISA KitHamster DPD ELISA KitHorse DPD ELISA KitMonkey DPD ELISA KitSheep DPD ELISA Kit
  • SpecificityThis kit recognizes natural and recombinant Mouse DPD. No significant cross-reactivity or interference between Mouse DPD and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of Mouse DPD is 1.875ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
  • Intended UseMouse DPD ELISA Kit allows for the in vitro quantitative determination of DPD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with DPD. During the reaction, DPD in the sample or standard competes with a fixed amount of DPD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DPD. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of DPD in the samples is then determined by comparing the OD of the samples to the standard curve.


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