Mouse DAT ELISA Kit

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  • Alternative name

    Sodium-dependent dopamine transporter ELISA KIT; Solute carrier family 6 member 3SLC6A3 ELISA KIT; DAT1 ELISA KIT; DA transporter ELISA KIT; DAT ELISA KIT

  • Catalog
    E051244
  • species
    Mouse
  • GeneDAT
  • Standard CurveMouse DAT ELISA Kit
  • Other Species Human DAT ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of DAT. No significant cross-reactivity or interference between DAT and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of DAT is typically less than 0.255ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two
  • Intended UseMouse DAT ELISA Kit allows for the in vitro quantitative determination of DAT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DAT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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