Mouse Ddit3 ELISA Kit

Alternative name
Mouse DDIT-3 ELISA Kit;Mouse C/EBP zeta ELISA Kit;Mouse C/EBP-homologous protein ELISA Kit;Mouse chop ELISA Kit;Mouse c/EBP-homologous protein 10 ELISA Kit;Mouse CHOP-10 ELISA Kit;Mouse CCAAT/enhancer-binding protein homologous protein ELISA Kit;Mouse growth arrest and DNA-damage-inducible protein GADD153 ELISA Kit;Mouse CHOP10 ELISA Kit;Mouse gadd153 ELISA Kit;Mouse DNA-damage inducible transcript 3 ELISA Kit;Mouse DNA damage-inducible transcript 3 protein ELISA Kit;Mouse C/EBP homoologous protein 10 ELISA Kit;
Catalog
E051098
species
Mouse
GeneDdit3
SpecificityThis assay has high sensitivity and excellent specificity for detection of DNA Damage Inducible Transcript 3 (DDIT3). No significant cross-reactivity or interference between DNA Damage Inducible Transcript 3 (DDIT3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.058ng/mL.
Intended UseMouse Ddit3 ELISA Kit allows for the in vitro quantitative determination of Ddit3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA Damage Inducible Transcript 3 (DDIT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DNA Damage Inducible Transcript 3 (DDIT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNA Damage Inducible Transcript 3 (DDIT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DNA Damage Inducible Transcript 3 (DDIT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse DNA damage-inducible transcript 3 protein Protein information
Uniprot ID DDIT3_MOUSE
Uniprot AC P35639; Q91YW9;
UniGene Mm.110220;
GeneID 13198
KEGG mmu:13198;
Mouse DNA damage-inducible transcript 3 protein Protein SEQUENCE
SEQUENCE 168 AA; 19189 MW; CA423B79512F33AB CRC64;
MAAESLPFTL ETVSSWELEA WYEDLQEVLS SDEIGGTYIS SPGNEEEESK
TFTTLDPASL AWLTEEPGPT EVTRTSQSPR SPDSSQSSMA QEEEEEEQGR
TRKRKQSGQC PARPGKQRMK EKEQENERKV AQLAEENERL KQEIERLTRE
VETTRRALID RMVSLHQA
UCSC uc007hiy.2; mouse.;

Mouse Ddit3 ELISA Kit

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