Human PRG2 ELISA Kit

Alternative name
Human BMPG ELISA Kit;Human Proteoglycan 2 ELISA Kit;Human EMBP ELISA Kit;Human MBP ELISA Kit;Human Pregnancy-associated major basic protein ELISA Kit;Human MBP1 ELISA Kit;Human proMBP ELISA Kit;Human proteoglycan 2, pro eosinophil major basic protein ELISA Kit;Human bone marrow proteoglycan ELISA Kit;Human eosinophil granule major basic protein ELISA Kit;Human eosinophil major basic protein ELISA Kit;Human natural killer cell activator ELISA Kit;Human proteoglycan 2 preproprotein ELISA Kit;Human proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein) ELISA Kit;
Catalog
E005090
species
Human
GenePRG2
SpecificityThis assay has high sensitivity and excellent specificity for detection of PRG2. No significant cross-reactivity or interference between PRG2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PRG2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman PRG2 ELISA Kit allows for the in vitro quantitative determination of PRG2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the assay: PRG2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PRG2 antibody and an PRG2-HRP conjugate. The assay sample and buffer are incubated together with PRG2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRG2 concentration since PRG2 from samples and PRG2-HRP conjugate compete for the anti-PRG2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PRG2 from the sample, fewer sites are left to bind PRG2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRG2 concentration in each sample is interpolated from this standard curve.
Human Eosinophil granule major basic protein Protein information
Uniprot ID PRG2_HUMAN
Uniprot AC P13727; A6XMW0; B2R5I1; P81448; Q14227; Q6ICT2;
UniGene Hs.512633;
GeneID 5553
KEGG hsa:5553;
Human Eosinophil granule major basic protein Protein SEQUENCE
SEQUENCE 222 AA; 25206 MW; CDD545642555E2D0 CRC64;
MKLPLLLALL FGAVSALHLR SETSTFETPL GAKTLPEDEE TPEQEMEETP
CRELEEEEEW GSGSEDASKK DGAVESISVP DMVDKNLTCP EEEDTVKVVG
IPGCQTCRYL LVRSLQTFSQ AWFTCRRCYR GNLVSIHNFN INYRIQCSVS
ALNQGQVWIG GRITGSGRCR RFQWVDGSRW NFAYWAAHQP WSRGGHCVAL
CTRGGHWRRA HCLRRLPFIC SY
UCSC uc001nkc.4; human. [P13727-1];

Human PRG2 ELISA Kit

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