Human PRG2 ELISA Kit

Contact Us
  • Alternative name

    Human BMPG ELISA Kit;Human Proteoglycan 2 ELISA Kit;Human EMBP ELISA Kit;Human MBP ELISA Kit;Human Pregnancy-associated major basic protein ELISA Kit;Human MBP1 ELISA Kit;Human proMBP ELISA Kit;Human proteoglycan 2, pro eosinophil major basic protein ELISA Kit;Human bone marrow proteoglycan ELISA Kit;Human eosinophil granule major basic protein ELISA Kit;Human eosinophil major basic protein ELISA Kit;Human natural killer cell activator ELISA Kit;Human proteoglycan 2 preproprotein ELISA Kit;Human proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein) ELISA Kit;

  • Catalog
    E005090
  • species
    Human
  • GenePRG2
  • Standard CurveHuman PRG2 ELISA Kit
  • Other Species Mouse Prg2 ELISA KitRat Prg2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PRG2. No significant cross-reactivity or interference between PRG2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PRG2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman PRG2 ELISA Kit allows for the in vitro quantitative determination of PRG2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the assay: PRG2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PRG2 antibody and an PRG2-HRP conjugate. The assay sample and buffer are incubated together with PRG2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRG2 concentration since PRG2 from samples and PRG2-HRP conjugate compete for the anti-PRG2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PRG2 from the sample, fewer sites are left to bind PRG2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRG2 concentration in each sample is interpolated from this standard curve.
  • Human Eosinophil granule major basic protein Protein information
  • Uniprot ID PRG2_HUMAN
  • Uniprot AC P13727; A6XMW0; B2R5I1; P81448; Q14227; Q6ICT2;
  • UniGene Hs.512633;
  • GeneID 5553
  • KEGG hsa:5553;
  • Human Eosinophil granule major basic protein Protein SEQUENCE
  • SEQUENCE 222 AA; 25206 MW; CDD545642555E2D0 CRC64;

    MKLPLLLALL FGAVSALHLR SETSTFETPL GAKTLPEDEE TPEQEMEETP

    CRELEEEEEW GSGSEDASKK DGAVESISVP DMVDKNLTCP EEEDTVKVVG

    IPGCQTCRYL LVRSLQTFSQ AWFTCRRCYR GNLVSIHNFN INYRIQCSVS

    ALNQGQVWIG GRITGSGRCR RFQWVDGSRW NFAYWAAHQP WSRGGHCVAL

    CTRGGHWRRA HCLRRLPFIC SY

  • UCSC uc001nkc.4; human. [P13727-1];


Related products with Human PRG2 ELISA Kit


tag

Inquiry About Human PRG2 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!