Mouse Dpt ELISA Kit

Alternative name
Mouse Early quiescence protein 1 ELISA Kit;Mouse EQ-1 ELISA Kit;Mouse tyrosine-rich acidic matrix protein ELISA Kit;Mouse TRAMP ELISA Kit;Mouse 1810032B19Rik ELISA Kit;Mouse 5033416F05Rik ELISA Kit;Mouse Eq1 ELISA Kit;Mouse dermatopontin ELISA Kit;Mouse early quiescence protein-1 ELISA Kit;
Catalog
E050810
species
Mouse
GeneDpt
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Dpt ELISA Kit allows for the in vitro quantitative determination of Dpt , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This DPT ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofDPT in the sample, thisDPT ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusDPT concentration. The concentration ofDPT in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This DPT enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forDPT. Standards or samples are then added to the microtiter plate wells andDPT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofDPT present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forDPT are added to each well to "sandwich" theDPT immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containDPT and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
Mouse Dermatopontin Protein information
Uniprot ID DERM_MOUSE
Uniprot AC Q9QZZ6; Q9D2B6;
UniGene Mm.28935;
GeneID 56429
KEGG mmu:56429;
Mouse Dermatopontin Protein SEQUENCE
SEQUENCE 201 AA; 23995 MW; DA64550025B9C98E CRC64;
MDLTLLWVLL PLVTTAWGQY GGYGYPYQQY QDYGDDGWVN LNRQGFSYQC
PHGQVVVAVR SIFSKKEGSD RQWNYACMPT PQSLGEPTEC WWEEINRAGM
EWYQKCSNNG LVAGFQSRYF ESVLDREWQF YCCRYSKRCP YSCWMTTEYP
SHYGEDMDMI SYDYDFYMRG ATTTFSAVER DRQWKFIMCR MTDYDCEFEN
V
UCSC uc007diq.1; mouse.;

Category

Mouse Dpt ELISA Kit

Mouse Early quiescence protein 1 ELISA Kit;Mouse EQ-1 ELISA Kit;Mouse tyrosine-rich acidic matrix protein ELISA Kit;Mouse TRAMP ELISA Kit;Mouse 1810032B19Rik ELISA Kit;Mouse 5033416F05Rik ELISA Kit;Mouse Eq1 ELISA Kit;Mouse dermatopontin ELISA Kit;Mouse early quiescence protein-1 ELISA Kit;