Mouse Dsp ELISA Kit

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  • Alternative name

    Mouse DP ELISA Kit;Mouse 2300002E22Rik ELISA Kit;Mouse 5730453H04Rik ELISA Kit;Mouse AA407887 ELISA Kit;Mouse AA407888 ELISA Kit;Mouse AW109828 ELISA Kit;Mouse rul ELISA Kit;Mouse desmoplakin ELISA Kit;Mouse desmoplakin I/II ELISA Kit;Mouse desmosomal cytoskeletal connector molecule ELISA Kit;

  • Catalog
    E050738
  • species
    Mouse
  • GeneDsp
  • Standard CurveMouse Dsp ELISA Kit
  • Other Species Human DSP ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseMouse Dsp ELISA Kit allows for the in vitro quantitative determination of Dsp , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This DSP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse DSP. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY DSP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DSP antibody and an DSP-HRP conjugate. The assay sample and buffer are incubated together with DSP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DSP concentration since DSP from samples and DSP-HRP conjugate compete for the anti-DSP antibody binding site. Since the number of sites is limited, as more sites are occupied by DSP from the sample, fewer sites are left to bind DSP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DSP concentration in each sample is interpolated from this standard curve.
  • Mouse Desmoplakin Protein information
  • Uniprot ID DESP_MOUSE
  • Uniprot AC E9Q557;
  • UniGene Mm.355327;
  • GeneID 109620
  • KEGG mmu:109620;
  • Mouse Desmoplakin Protein SEQUENCE
  • SEQUENCE 2883 AA; 332912 MW; 1D8B68788D519940 CRC64;

    MSCNGGSHPR INTLGRMTRA ESGPDLRYEM TYSGGGGGGG GGGGGGTSRT

    FYSHSRRCTV NDQNSDGYCQ TGTMSRHQNQ NTIQEMLQNC SDCLMRAELI

    AQPELKFGEG MQLAWNRELD EYFTQANDQM EIIDGLIREM RQMGQPCDAY

    QKRLLQLQEQ MRALYKAISV PRVRRASSKG AGGYTCQSGS GWDEFTKRLT

    GECLGWMRQQ REEMDLMAWG VDAGSVEQHI NSHRSIHNTI GDYRWQLDKI

    KADLREKSAI YQLEEEYENL LKASFERMDH LRQLQNIIQA TSREIMWIND

    CEEEELLYDW SDKNTNIAQK QEAFSIRMSQ LEVKEKELNK LKQESDQLVL

    NQHPASDKIE AYMDTLQTQW SWILQITKCI DVHLKENAAY FQFFEEAQST

    EAYLKGLQDS IRKKYPCDKN MPLQHLLEQI KELEKEREKI IEYKRQVQNL

    VNKSKKIVQL KPRNPDYRSN KPIILRALCD YKQDQKIVHK GDECILKDNN

    ERSKWYVTGP GGVDMLVPSV GLIIPPPNPL AVDLSCKIEQ YYEAILALWN

    QLYINMKSLV SWHYCMIDIE KIRAMTIAKL KTMRQEDYMK TIEDLELHYQ

    DFIKNSQGSE MFGDDDKRRM QSQFTDAQKH YQTLVIQLPG HPQHQTVTKT

    EITHLGTCQD VNHNKVIETN RENDKQETWL LMELQKIRRQ MEHCEARMTL

    KNLLLAEQGS THHITVKINE LKSVQNDSQA LAEVLNQLKD MLANFRGSEK

    YCYLQNEIFG LFQKLENING VSDGYLNSLC SVRALLQAIL QTEDMLKVYE

    ARLTEEETVC LDLDKVEAYR CGLKKIKNDL NLKKSLLATM KTELQKAQQI

    HSQSSQQYPL YDLDLGKFTE KVTQLTDRWQ KIDKQIDFRL WDLEKQIKQL

    RNYRDNYQSF CKWLYDAKRR QDSLESMKFG DSNTVMRFLN EQKNLHSEIS

    GKRDKSEEVH KIAELCANSI KDYELQLASY TSGLETLLNI PIKRTMVQSP

    SGVILQEAAD IHARYIELLT RSGDYYRFLS EMLKSLEDLK LKNTKIEVLE

    EELRLARDAN SENCNKNKFL DQNLQKYQAE CSQFKAKLVS LEELKRQAEL

    DGKSAKQNLD KCYGQIKELN EKITRLTYEI EDEKRRRKTV EDRFDQQKND

    YDQLQKARQC EKENLSWQKL ESEKAIKEKE YEIERLRVLL QEEGARKREY

    ENELAKVRNH YNEEMSNLRN KYETEINITK TTIKEISMQK EDDSKNLRNQ

    MDRLSRENRD LKDEIVRLND SILQATEQRR RAEENALQQK ACGSETMQKK

    QRLEIELKQV IQQRSEDNAR HKQSLEEAAK TIQDKNKEIE RLKAEYQEEA

    KRRWEYENEL SKVRNSYDEE IISLKNQFET EINITKTTIH QLTMQKEEDT

    SGYRAQIDNL TRENRSLCEE VKRLKNTLAQ TTENLRRVEE NAQQQKATGS

    EMSQRKQQLE IELRQVTQMR TEESMRYKQS LDDAAKTIQD KNKEIERLKQ

    LVDKETNERK CLEDENSKLQ RVQYDLQKAN NSATEAMSKL KVQEQELTRL

    RIDYERVSQE RTVKDQDITR IQSSLKDLQL QKQKAEEELS RLKRTASDES

    SKRKMLEEEL EAMRRSLKEQ AVKITNLTQQ LEQASIVKKR SEDDLRQQRD

    VLDGHVREKQ RTQEELRRLS LDVEALRRQL VQEQENVKQA HLRNEHFQKA

    IEDKSRSLNE SKIEIERLQS LTENLTKEHL MLEEELRNLR LEYDDLRRGR

    SEADSDKNST ISELRSQLQI SNNRTLELQG LINDLQRERE NLRQEIEKFQ

    KQALEASNRI QESKSQCTQV VQERESLLVK IKVLEQDKAR LQRLEDELNR

    AKATLEAESR VKQRLECEKQ QIQNDLNQWK TQYSRKEETI RKIESEREKS

    EREKNSLRSE IERLQAEIKR IEERCRRKLE DSSRETQSQL ESERCRLQKE

    IEKLRQRPYG SHRETQTEYE WTVDSSKLVF DGLRKKVTAM QLYECQLIDK

    TTLDKLLKGK KSVEEVASEI QPFLRGAGAI AGASASPKEK YSLVEAKRKK

    FITPESTVML LEAQAATGGI IDPHRNEKLT VDNAVARDLI DFDDRQQIYT

    AEKAITGFDD PFSGKTVSVS EAIKKNLIDR ETGMRLLEAQ LASGGVVDPV

    NSVFLPKDVA LARGLIDRDL YRSLNDPRDS QKNFVDPITK KKVSYMQLRE

    RCRIEPHTGL LLLSVQKRSM SFQGIRQPVT VTELVDSGIL RPSTVNELES

    GQISYDEVGE RIKDFLQGSS CIAGIYNETT KQKLGIYEAM KIGLVRPGTA

    LELLEAQAAT GFIVDPVSNL RLPVEEAYKR GLVGIEFKEK LLSAERAVTG

    YNDPETGNII SLFQAMNKEL IEKGHGIRLL EAQIATGGII DPKESHRLPV

    DMAYKRGYFN EELSEILSDP SDDTKGFFDP NTEENLTYLQ LKERCIKDEE

    TGLCLLPLKE KKKQVQTSQK NTLRKRRVVI VDPETNKEMS VQEAYKKGLI

    DYDTFKELCE QECEWEEITI TGSDGSTRVV LVDRKTGSQY DIQDAIDKGL

    VDRKFFDQYR SGSLSLTQFA DMISLKNGVG NSSGLGGSVN DDVFSSSRHD

    SVSKISTISS VRNLTIRSSS LSDPLEESSP IAAIFDTENL EKISIAEGIE

    RGIVDSITGQ RLLEAQACTG GIIHPTTGQK LSLQDAVNQG LIDQDMATRL

    KPAQKAFIGF EGVKGKKKMS AAEAVKEKWL PYEAGQRFLE FQFLTGGLVD

    PEVHGRISTE EAIRKGFIDG RAAQRLQDIS SYAKILTCPK TKLKISYKDA

    MNRSMVEDIT GLRLLEAASV SSKGLPSPYN MSAPGSRSGS RSGSRSGSRS

    GSRSGSRRGS FDATGNSSYS YSYSFSSSSI GGY

  • UCSC uc007qdk.2; mouse.;


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