Mouse Ech1 ELISA Kit

Alternative name
Mouse AA617331 ELISA Kit;Mouse enoyl coenzyme A hydratase 1, peroxisomal ELISA Kit;Mouse delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial ELISA Kit;Mouse dienoyl-CoA isomerase ELISA Kit;Mouse peroxisomal/mitochondrial dienoyl-CoA isomerase ELISA Kit;
Catalog
E050695
species
Mouse
GeneEch1
SpecificityThis assay recognizes recombinant and natural Mouse Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial. No significant cross-reactivity or interference was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Ech1 ELISA Kit allows for the in vitro quantitative determination of Ech1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial, During the reaction, Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial in the sample or standard competes with a fixed amount of biotin-labeled Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial for sites on a pre-coated Monoclonal antibody specific to Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial Protein information
Uniprot ID ECH1_MOUSE
Uniprot AC O35459; Q5M8P6;
UniGene Mm.291776;
GeneID 51798
KEGG mmu:51798;
Mouse Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial Protein SEQUENCE
SEQUENCE 327 AA; 36118 MW; CC0E612C74FBEE2E CRC64;
MATAMTVSSK LRGLLMQQLR GTSQLYFNIS LRSLSSSAQE ASKRAPEEVS
DHNYESIQVT SAQKHVLHVQ LNRPEKRNAM NRAFWRELVE CFQKISKDSD
CRAVVVSGAG KMFTSGIDLM DMASELMQPS GDDAARIAWY LRDLISKYQK
TFTVIEKCPK PVIAAIHGGC IGGGVDLVSA CDIRYCTQDA FFQIKEVDMG
LAADVGTLQR LPKVIGNQSL VNELTFSARK MMADEALDSG LVSRVFQDKD
AMLNAAFALA ADISSKSPVA VQGSKINLIY SRDHSVDESL DYMATWNMSM
LQTQDIIKSV QAAMEKRDTK SITFSKL
UCSC uc009gab.1; mouse.;

Category

Mouse Ech1 ELISA Kit

Mouse AA617331 ELISA Kit;Mouse enoyl coenzyme A hydratase 1, peroxisomal ELISA Kit;Mouse delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial ELISA Kit;Mouse dienoyl-CoA isomerase ELISA Kit;Mouse peroxisomal/mitochondrial dienoyl-CoA isomerase ELISA Kit;