Mouse Dio2 ELISA Kit

Alternative name
Mouse 5DII ELISA Kit;Mouse DIOII ELISA Kit;Mouse type 2 DI ELISA Kit;Mouse Type-II 5'-deiodinase ELISA Kit;Mouse AI324267 ELISA Kit;Mouse deiodinase, iodothyronine, type II ELISA Kit;Mouse type II iodothyronine deiodinase ELISA Kit;Mouse iodothyronine type II deiodinase ELISA Kit;Mouse type-II 5' deiodinase ELISA Kit;
Catalog
E050677
species
Mouse
GeneDio2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Deiodinase, Iodothyronine, Type II (DIO2). No significant cross-reactivity or interference between Deiodinase, Iodothyronine, Type II (DIO2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.28ng/mL.
Intended UseMouse Dio2 ELISA Kit allows for the in vitro quantitative determination of Dio2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Deiodinase, Iodothyronine, Type II (DIO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Deiodinase, Iodothyronine, Type II (DIO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Deiodinase, Iodothyronine, Type II (DIO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Deiodinase, Iodothyronine, Type II (DIO2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Type II iodothyronine deiodinase Protein information
Uniprot ID IOD2_MOUSE
Uniprot AC Q9Z1Y9; Q05A70; Q9JHH1;
UniGene Mm.21389; Mm.408815;
GeneID 13371
KEGG mmu:13371;
Mouse Type II iodothyronine deiodinase Protein SEQUENCE
SEQUENCE 266 AA; 29929 MW; B2FD28E171DC5486 CRC64;
MGLLSVDLLI TLQILPVFFS NCLFLALYDS VILLKHVALL LSRSKSTRGE
WRRMLTSEGL RCVWNSFLLD AYKQVKLGED APNSSVVHVS NPESGNNYAS
EKTADGAECH LLDFASAERP LVVNFGSATU PPFTRQLPAF RQLVEEFSSV
ADFLLVYIDE AHPSDGWAVP GDSSLSFEVK KHRNQEDRCA AAHQLLERFS
LPPQCQVVAD RMDNNANVAY GVAFERVCIV QRRKIAYLGG KGPFSYNLQE
VRSWLEKNFS KRUILD
UCSC uc007okf.1; mouse.;

Mouse Dio2 ELISA Kit

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