SensitivityThe minimum detectable dose of mouse DHEA is typically less than 0.1 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest mouse DHEA concentration that could be differentiated from zero. It was determined the m
Intended UseMouse DHEA ELISA Kit allows for the in vitro quantitative determination of DHEA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for DHEA and Horseradish Peroxidase (HRP) conjugated DHEA. The competitive inhibition reaction is launched between with HRP labeled DHEA and unlabeled DHEA with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of DHEA in the sample. The color development is stopped and the intensity of the color is measured.