Human BMP7 ELISA Kit

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  • Alternative name

    Human BMP-7 ELISA Kit;Human osteogenic protein 1 ELISA Kit;Human OP-1 ELISA Kit;Human OP1 ELISA Kit;Human bone morphogenetic protein 7 ELISA Kit;

  • Catalog
    E005062
  • species
    Human
  • GeneBMP7
  • Standard CurveHuman BMP7 ELISA Kit
  • Other Species Mouse Bmp7 ELISA KitCanine BMP7 ELISA Kit
  • SpecificityNatural and recombinant human BMP-7
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman BMP7 ELISA Kit allows for the in vitro quantitative determination of BMP7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human BMP-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-7 has been precoated onto 96-well plates. Standards(NSO, S293-H431) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BMP-7 amount of sample captured in plate. Background: Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.
  • Human Bone morphogenetic protein 7 Protein information
  • Uniprot ID BMP7_HUMAN
  • Uniprot AC P18075; Q9H512; Q9NTQ7;
  • UniGene Hs.473163;
  • GeneID 655
  • KEGG hsa:655;
  • Human Bone morphogenetic protein 7 Protein SEQUENCE
  • SEQUENCE 431 AA; 49313 MW; 47A05E45C6815F8A CRC64;

    MHVRSLRAAA PHSFVALWAP LFLLRSALAD FSLDNEVHSS FIHRRLRSQE

    RREMQREILS ILGLPHRPRP HLQGKHNSAP MFMLDLYNAM AVEEGGGPGG

    QGFSYPYKAV FSTQGPPLAS LQDSHFLTDA DMVMSFVNLV EHDKEFFHPR

    YHHREFRFDL SKIPEGEAVT AAEFRIYKDY IRERFDNETF RISVYQVLQE

    HLGRESDLFL LDSRTLWASE EGWLVFDITA TSNHWVVNPR HNLGLQLSVE

    TLDGQSINPK LAGLIGRHGP QNKQPFMVAF FKATEVHFRS IRSTGSKQRS

    QNRSKTPKNQ EALRMANVAE NSSSDQRQAC KKHELYVSFR DLGWQDWIIA

    PEGYAAYYCE GECAFPLNSY MNATNHAIVQ TLVHFINPET VPKPCCAPTQ

    LNAISVLYFD DSSNVILKKY RNMVVRACGC H

  • UCSC uc010gip.2; human.;




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