Human BMP5 ELISA Kit

Alternative name
Human BMP-5 ELISA Kit;Human bone morphogenetic protein 5 ELISA Kit;
Catalog
E005059
species
Human
GeneBMP5
Standard Curve
Other Species Mouse Bmp5 ELISA Kit
SpecificityNatural and recombinant human BMP-5
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman BMP5 ELISA Kit allows for the in vitro quantitative determination of BMP5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human BMP-5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-5 has been precoated onto 96-well plates. Standards(NSO, A317-H454) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BMP-5 amount of sample captured in plate. Background: Bone morphogenetic protein 5 is a protein that in humans is encoded by the BMP5 gene.1,2,3 The protein encodedby this gene is member of the TGFbeta superfamily. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP5 may play a role in certain cancers. Like other BMP's BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. BMP-5 is mapped on human chromosome 6.4 The standard used in this kit is recombinant BMP-5 with the monomer molecular mass of18KDa.
Human Bone morphogenetic protein 5 Protein information
Uniprot ID BMP5_HUMAN
Uniprot AC P22003; B4E0Y4; Q9H547; Q9NTM5;
UniGene Hs.296648; Hs.656729;
GeneID 653
KEGG hsa:653;
Human Bone morphogenetic protein 5 Protein SEQUENCE
SEQUENCE 454 AA; 51737 MW; 631277413CCC22EE CRC64;
MHLTVFLLKG IVGFLWSCWV LVGYAKGGLG DNHVHSSFIY RRLRNHERRE
IQREILSILG LPHRPRPFSP GKQASSAPLF MLDLYNAMTN EENPEESEYS
VRASLAEETR GARKGYPASP NGYPRRIQLS RTTPLTTQSP PLASLHDTNF
LNDADMVMSF VNLVERDKDF SHQRRHYKEF RFDLTQIPHG EAVTAAEFRI
YKDRSNNRFE NETIKISIYQ IIKEYTNRDA DLFLLDTRKA QALDVGWLVF
DITVTSNHWV INPQNNLGLQ LCAETGDGRS INVKSAGLVG RQGPQSKQPF
MVAFFKASEV LLRSVRAANK RKNQNRNKSS SHQDSSRMSS VGDYNTSEQK
QACKKHELYV SFRDLGWQDW IIAPEGYAAF YCDGECSFPL NAHMNATNHA
IVQTLVHLMF PDHVPKPCCA PTKLNAISVL YFDDSSNVIL KKYRNMVVRS
CGCH
UCSC uc003pcq.4; human. [P22003-1];

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Category

Human BMP5 ELISA Kit

Human BMP-5 ELISA Kit;Human bone morphogenetic protein 5 ELISA Kit;