Human BMP4 ELISA Kit

Alternative name
Human BMP-4 ELISA Kit;Human bone morphogenetic protein 2B ELISA Kit;Human BMP-2B ELISA Kit;Human BMP2B ELISA Kit;Human DVR4 ELISA Kit;Human BMP2B1 ELISA Kit;Human MCOPS6 ELISA Kit;Human OFC11 ELISA Kit;Human ZYME ELISA Kit;Human bone morphogenetic protein 4 ELISA Kit;
Catalog
E005057
species
Human
GeneBMP4
Standard Curve
Other Species Mouse Bmp4 ELISA Kit Bovine BMP4 ELISA Kit Chicken BMP4 ELISA Kit Rabbit BMP4 ELISA Kit Rat Bmp4 ELISA Kit
SpecificityNatural and recombinant human BMP-4
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 4pg/ml
Intended UseHuman BMP4 ELISA Kit allows for the in vitro quantitative determination of BMP4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human BMP-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-4 has been precoated onto 96-well plates. Standards(NSO, S293-R408) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BMP-4 amount of sample captured in plate. Background: Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23.1, 2 The protein encoded by this gene is a member of the bone morphogenetic protein family which ispart of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. The standard product used in this kit is recombinant human BMP-4 with the molecular mass of 26KDa.
Human Bone morphogenetic protein 4 Protein information
Uniprot ID BMP4_HUMAN
Uniprot AC P12644; Q9UM80;
UniGene Hs.68879;
GeneID 652
KEGG hsa:652;
Human Bone morphogenetic protein 4 Protein SEQUENCE
SEQUENCE 408 AA; 46555 MW; 79B01179DBB98204 CRC64;
MIPGNRMLMV VLLCQVLLGG ASHASLIPET GKKKVAEIQG HAGGRRSGQS
HELLRDFEAT LLQMFGLRRR PQPSKSAVIP DYMRDLYRLQ SGEEEEEQIH
STGLEYPERP ASRANTVRSF HHEEHLENIP GTSENSAFRF LFNLSSIPEN
EVISSAELRL FREQVDQGPD WERGFHRINI YEVMKPPAEV VPGHLITRLL
DTRLVHHNVT RWETFDVSPA VLRWTREKQP NYGLAIEVTH LHQTRTHQGQ
HVRISRSLPQ GSGNWAQLRP LLVTFGHDGR GHALTRRRRA KRSPKHHSQR
ARKKNKNCRR HSLYVDFSDV GWNDWIVAPP GYQAFYCHGD CPFPLADHLN
STNHAIVQTL VNSVNSSIPK ACCVPTELSA ISMLYLDEYD KVVLKNYQEM
VVEGCGCR

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Category

Human BMP4 ELISA Kit

Human BMP-4 ELISA Kit;Human bone morphogenetic protein 2B ELISA Kit;Human BMP-2B ELISA Kit;Human BMP2B ELISA Kit;Human DVR4 ELISA Kit;Human BMP2B1 ELISA Kit;Human MCOPS6 ELISA Kit;Human OFC11 ELISA Kit;Human ZYME ELISA Kit;Human bone morphogenetic protein 4 ELISA Kit;