Human BMP2 ELISA Kit

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  • Alternative name

    Human BMP-2 ELISA Kit;Human bone morphogenetic protein 2A ELISA Kit;Human BMP-2A ELISA Kit;Human BMP2A ELISA Kit;Human BDA2 ELISA Kit;Human bone morphogenetic protein 2 ELISA Kit;

  • Catalog
    E005052
  • species
    Human
  • GeneBMP2
  • Standard CurveHuman BMP2 ELISA Kit
  • Other Species Mouse Bmp2 ELISA KitChicken BMP2 ELISA KitRabbit BMP2 ELISA KitRat Bmp2 ELISA Kit
  • SpecificityNatural and recombinant human BMP-2
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 2pg/ml
  • Intended UseHuman BMP2 ELISA Kit allows for the in vitro quantitative determination of BMP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-2 has been precoated onto 96-well plates. Standards(CHO,Q283-R396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BMP-2 amount of sample captured in plate. Background: Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein inducesmedulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinicaltrials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant human BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
  • Human Bone morphogenetic protein 2 Protein information
  • Uniprot ID BMP2_HUMAN
  • Uniprot AC P12643;
  • UniGene Hs.73853;
  • GeneID 650
  • KEGG hsa:650;
  • Human Bone morphogenetic protein 2 Protein SEQUENCE
  • SEQUENCE 396 AA; 44702 MW; 20653A3987B25E60 CRC64;

    MVAGTRCLLA LLLPQVLLGG AAGLVPELGR RKFAAASSGR PSSQPSDEVL

    SEFELRLLSM FGLKQRPTPS RDAVVPPYML DLYRRHSGQP GSPAPDHRLE

    RAASRANTVR SFHHEESLEE LPETSGKTTR RFFFNLSSIP TEEFITSAEL

    QVFREQMQDA LGNNSSFHHR INIYEIIKPA TANSKFPVTR LLDTRLVNQN

    ASRWESFDVT PAVMRWTAQG HANHGFVVEV AHLEEKQGVS KRHVRISRSL

    HQDEHSWSQI RPLLVTFGHD GKGHPLHKRE KRQAKHKQRK RLKSSCKRHP

    LYVDFSDVGW NDWIVAPPGY HAFYCHGECP FPLADHLNST NHAIVQTLVN

    SVNSKIPKAC CVPTELSAIS MLYLDENEKV VLKNYQDMVV EGCGCR

  • UCSC uc002wmu.2; human.;




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