Human BMP2 ELISA Kit

Alternative name
Human BMP-2 ELISA Kit;Human bone morphogenetic protein 2A ELISA Kit;Human BMP-2A ELISA Kit;Human BMP2A ELISA Kit;Human BDA2 ELISA Kit;Human bone morphogenetic protein 2 ELISA Kit;
Catalog
E005052
species
Human
GeneBMP2
Standard Curve
Other Species Mouse Bmp2 ELISA Kit Chicken BMP2 ELISA Kit Rabbit BMP2 ELISA Kit Rat Bmp2 ELISA Kit
SpecificityNatural and recombinant human BMP-2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 2pg/ml
Intended UseHuman BMP2 ELISA Kit allows for the in vitro quantitative determination of BMP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-2 has been precoated onto 96-well plates. Standards(CHO,Q283-R396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BMP-2 amount of sample captured in plate. Background: Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein inducesmedulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinicaltrials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant human BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
Human Bone morphogenetic protein 2 Protein information
Uniprot ID BMP2_HUMAN
Uniprot AC P12643;
UniGene Hs.73853;
GeneID 650
KEGG hsa:650;
Human Bone morphogenetic protein 2 Protein SEQUENCE
SEQUENCE 396 AA; 44702 MW; 20653A3987B25E60 CRC64;
MVAGTRCLLA LLLPQVLLGG AAGLVPELGR RKFAAASSGR PSSQPSDEVL
SEFELRLLSM FGLKQRPTPS RDAVVPPYML DLYRRHSGQP GSPAPDHRLE
RAASRANTVR SFHHEESLEE LPETSGKTTR RFFFNLSSIP TEEFITSAEL
QVFREQMQDA LGNNSSFHHR INIYEIIKPA TANSKFPVTR LLDTRLVNQN
ASRWESFDVT PAVMRWTAQG HANHGFVVEV AHLEEKQGVS KRHVRISRSL
HQDEHSWSQI RPLLVTFGHD GKGHPLHKRE KRQAKHKQRK RLKSSCKRHP
LYVDFSDVGW NDWIVAPPGY HAFYCHGECP FPLADHLNST NHAIVQTLVN
SVNSKIPKAC CVPTELSAIS MLYLDENEKV VLKNYQDMVV EGCGCR
UCSC uc002wmu.2; human.;

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Category

Human BMP2 ELISA Kit

Human BMP-2 ELISA Kit;Human bone morphogenetic protein 2A ELISA Kit;Human BMP-2A ELISA Kit;Human BMP2A ELISA Kit;Human BDA2 ELISA Kit;Human bone morphogenetic protein 2 ELISA Kit;