Human AICDA ELISA Kit

Alternative name
Human Activation-induced cytidine deaminase ELISA Kit;Human AID ELISA Kit;Human cytidine aminohydrolase ELISA Kit;Human ARP2 ELISA Kit;Human CDA2 ELISA Kit;Human HEL-S-284 ELISA Kit;Human HIGM2 ELISA Kit;Human activation induced cytidine deaminase ELISA Kit;Human single-stranded DNA cytosine deaminase ELISA Kit;Human epididymis secretory protein Li 284 ELISA Kit;Human integrated into Burkitt's lymphoma cell line Ramos ELISA Kit;
Catalog
E000505
species
Human
GeneAICDA
SpecificityThis assay has high sensitivity and excellent specificity for detection of AICDA. No significant cross-reactivity or interference between AICDA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AICDA and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman AICDA ELISA Kit allows for the in vitro quantitative determination of AICDA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description
specificalIntended Uses: This AICDA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AICDA. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||AICDA ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-AICDA antibody and an AICDA-HRP conjugate. The assay sample and buffer are incubated together with AICDA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AICDA concentration since AICDA from samples and AICDA-HRP conjugate compete for the anti-AICDA antibody binding site. Since the number of sites is limited, as more sites are occupied by AICDA from the sample, fewer sites are left to bind AICDA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AICDA concentration in each sample is interpolated from this standard curve.
Human Single-stranded DNA cytosine deaminase Protein information
Uniprot ID AICDA_HUMAN
Uniprot AC Q9GZX7; Q6QJ81; Q8NFC1;
UniGene Hs.149342;
GeneID 57379
KEGG hsa:57379;
Human Single-stranded DNA cytosine deaminase Protein SEQUENCE
SEQUENCE 198 AA; 23954 MW; 3C27BB143DB184A9 CRC64;
MDSLLMNRRK FLYQFKNVRW AKGRRETYLC YVVKRRDSAT SFSLDFGYLR
NKNGCHVELL FLRYISDWDL DPGRCYRVTW FTSWSPCYDC ARHVADFLRG
NPNLSLRIFT ARLYFCEDRK AEPEGLRRLH RAGVQIAIMT FKDYFYCWNT
FVENHERTFK AWEGLHENSV RLSRQLRRIL LPLYEVDDLR DAFRTLGL
UCSC uc001qur.3; human. [Q9GZX7-1];

Human AICDA ELISA Kit

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