Human BMF ELISA Kit

Alternative name
Human Bcl2 modifying factor ELISA Kit;Human bcl-2-modifying factor ELISA Kit;
Catalog
E005037
species
Human
GeneBMF
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human BMF. No significant cross-reactivity or interference between Human BMF and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human BMF and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity56.25pg/mL
Intended UseHuman BMF ELISA Kit allows for the in vitro quantitative determination of BMF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BMF in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BMF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human BMF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human BMF. You can calculate the concentration of Human BMF in the samples by comparing the OD of the samples to the standard curve.
Human Bcl-2-modifying factor Protein information
Uniprot ID BMF_HUMAN
Uniprot AC Q96LC9; Q2M396; Q6NT30; Q6NT56; Q6P9F6; Q7Z7D4; Q7Z7D5; Q9H7K7;
UniGene Hs.591104;
GeneID 90427
KEGG hsa:90427;
Human Bcl-2-modifying factor Protein SEQUENCE
SEQUENCE 184 AA; 20508 MW; 21479B25CC727853 CRC64;
MEPSQCVEEL EDDVFQPEDG EPVTQPGSLL SADLFAQSLL DCPLSRLQLF
PLTHCCGPGL RPTSQEDKAT QTLSPASPSQ GVMLPCGVTE EPQRLFYGNA
GYRLPLPASF PAVLPIGEQP PEGQWQHQAE VQIARKLQCI ADQFHRLHVQ
QHQQNQNRVW WQILLFLHNL ALNGEENRNG AGPR
UCSC uc001zkt.6; human. [Q96LC9-1];

Human BMF ELISA Kit

Human Bcl2 modifying factor ELISA Kit;Human bcl-2-modifying factor ELISA Kit;

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