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Human BPGM ELISA Kit

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  • Alternative name

    Human BPGM ELISA Kit;Human 2,3-bisphosphoglycerate mutase, erythrocyte ELISA Kit;Human 2,3-bisphosphoglycerate synthase ELISA Kit;Human 2,3-diphosphoglycerate mutase ELISA Kit;Human DPGM ELISA Kit;Human BPG-dependent PGAM ELISA Kit;Human bisphosphoglycerate mutase ELISA Kit;Human erythrocyte 2,3-bisphosphoglycerate mutase ELISA Kit;Human testis secretory sperm-binding protein Li 202a ELISA Kit;
  • Catalog
    E005006
  • species
    Human
  • GeneBPGM
  • Standard CurveHuman BPGM ELISA Kit
  • Other Species Mouse Bpgm ELISA KitBovine BPGM ELISA KitMonkey BPGM ELISA KitRabbit BPGM ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of BPGM. No significant cross-reactivity or interference between BPGM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BPGM and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman BPGM ELISA Kit allows for the in vitro quantitative determination of BPGM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: BPGM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BPGM antibody and an BPGM-HRP conjugate. The assay sample and buffer are incubated together with BPGM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BPGM concentration since BPGM from samples and BPGM-HRP conjugate compete for the anti-BPGM antibody binding site. Since the number of sites is limited, as more sites are occupied by BPGM from the sample, fewer sites are left to bind BPGM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BPGM concentration in each sample is interpolated from this standard curve.
  • Human Bisphosphoglycerate mutase Protein information
  • Uniprot ID PMGE_HUMAN
  • Uniprot AC P07738; A4D1N9;
  • UniGene Hs.198365;
  • GeneID 669
  • KEGG hsa:669;
  • Human Bisphosphoglycerate mutase Protein SEQUENCE

  • SEQUENCE 259 AA; 30005 MW; A2AF1D6F2985A3B5 CRC64;

    MSKYKLIMLR HGEGAWNKEN RFCSWVDQKL NSEGMEEARN CGKQLKALNF

    EFDLVFTSVL NRSIHTAWLI LEELGQEWVP VESSWRLNER HYGALIGLNR

    EQMALNHGEE QVRLWRRSYN VTPPPIEESH PYYQEIYNDR RYKVCDVPLD

    QLPRSESLKD VLERLLPYWN ERIAPEVLRG KTILISAHGN SSRALLKHLE

    GISDEDIINI TLPTGVPILL ELDENLRAVG PHQFLGDQEA IQAAIKKVED

    QGKVKQAKK

  • UCSC uc003vrv.4; human.;


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