Human BPGM ELISA Kit

Alternative name
Human BPGM ELISA Kit;Human 2,3-bisphosphoglycerate mutase, erythrocyte ELISA Kit;Human 2,3-bisphosphoglycerate synthase ELISA Kit;Human 2,3-diphosphoglycerate mutase ELISA Kit;Human DPGM ELISA Kit;Human BPG-dependent PGAM ELISA Kit;Human bisphosphoglycerate mutase ELISA Kit;Human erythrocyte 2,3-bisphosphoglycerate mutase ELISA Kit;Human testis secretory sperm-binding protein Li 202a ELISA Kit;
Catalog
E005006
species
Human
GeneBPGM
SpecificityThis assay has high sensitivity and excellent specificity for detection of BPGM. No significant cross-reactivity or interference between BPGM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BPGM and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman BPGM ELISA Kit allows for the in vitro quantitative determination of BPGM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: BPGM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BPGM antibody and an BPGM-HRP conjugate. The assay sample and buffer are incubated together with BPGM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BPGM concentration since BPGM from samples and BPGM-HRP conjugate compete for the anti-BPGM antibody binding site. Since the number of sites is limited, as more sites are occupied by BPGM from the sample, fewer sites are left to bind BPGM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BPGM concentration in each sample is interpolated from this standard curve.
Human Bisphosphoglycerate mutase Protein information
Uniprot ID PMGE_HUMAN
Uniprot AC P07738; A4D1N9;
UniGene Hs.198365;
GeneID 669
KEGG hsa:669;
Human Bisphosphoglycerate mutase Protein SEQUENCE
SEQUENCE 259 AA; 30005 MW; A2AF1D6F2985A3B5 CRC64;
MSKYKLIMLR HGEGAWNKEN RFCSWVDQKL NSEGMEEARN CGKQLKALNF
EFDLVFTSVL NRSIHTAWLI LEELGQEWVP VESSWRLNER HYGALIGLNR
EQMALNHGEE QVRLWRRSYN VTPPPIEESH PYYQEIYNDR RYKVCDVPLD
QLPRSESLKD VLERLLPYWN ERIAPEVLRG KTILISAHGN SSRALLKHLE
GISDEDIINI TLPTGVPILL ELDENLRAVG PHQFLGDQEA IQAAIKKVED
QGKVKQAKK
UCSC uc003vrv.4; human.;

Human BPGM ELISA Kit

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