Mouse Cyp2e1 ELISA Kit

Alternative name
Mouse 4-nitrophenol 2-hydroxylase ELISA Kit;Mouse CYPIIE1 ELISA Kit;Mouse cytochrome P450-ALC ELISA Kit;Mouse cytochrome P450-J ELISA Kit;Mouse Cyp2e ELISA Kit;Mouse Cyp2e-1 ELISA Kit;Mouse cytochrome P450, family 2, subfamily e, polypeptide 1 ELISA Kit;Mouse cytochrome P450 2E1 ELISA Kit;Mouse cytochrome P450, 2e1, ethanol inducible ELISA Kit;
Catalog
E050058
species
Mouse
GeneCyp2e1
Other Species Human CYP2E1 ELISA Kit Monkey CYP2E1 ELISA Kit Rat Cyp2e1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CYP2E1. No significant cross-reactivity or interference between Mouse CYP2E1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CYP2E1 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.875ng/mL
Intended UseMouse Cyp2e1 ELISA Kit allows for the in vitro quantitative determination of Cyp2e1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CYP2E1 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CYP2E1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CYP2E1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CYP2E1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CYP2E1. You can calculate the concentration of Mouse CYP2E1 in the samples by comparing the OD of the samples to the standard curve.
Mouse Cytochrome P450 2E1 Protein information
Uniprot ID CP2E1_MOUSE
Uniprot AC Q05421; Q9Z198;
UniGene Mm.21758;
GeneID 13106
KEGG mmu:13106;
Mouse Cytochrome P450 2E1 Protein SEQUENCE
SEQUENCE 493 AA; 56805 MW; 4031AB016DA56A9C CRC64;
MAVLGITVAL LVWIATLLLV SIWKQIYRSW NLPPGPFPIP FFGNIFQLDL
KDIPKSLTKL AKRFGPVFTL HLGQRRIVVL HGYKAVKEVL LNHKNEFSGR
GDIPVFQEYK NKGIIFNNGP TWKDVRRFSL SILRDWGMGK QGNEARIQRE
AHFLVEELKK TKGQPFDPTF LIGCAPCNVI ADILFNKRFD YDDKKCLELM
SLFNENFYLL STPWIQAYNY FSDYLQYLPG SHRKVMKNVS EIRQYTLGKA
KEHLKSLDIN CPRDVTDCLL IEMEKEKHSQ EPMYTMENIS VTLADLFFAG
TETTSTTLRY GLLILMKYPE IEEKLHEEID RVIGPSRAPA VRDRMNMPYM
DAVVHEIQRF INLVPSNLPH EATRDTVFRG YVIPKGTVVI PTLDSLLFDN
YEFPDPETFK PEHFLNENGK FKYSDYFKAF SAGKRVCVGE GLARMELFLL
LSAILQHFNL KSLVDPKDID LSPVTIGFGS IPREFKLCVI PRS
UCSC uc009kic.1; mouse.;

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Mouse Cyp2e1 ELISA Kit