Human BTD ELISA Kit

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  • Alternative name

    Human biotinase ELISA Kit;Human biotinidase ELISA Kit;

  • Catalog
    E004999
  • species
    Human
  • GeneBTD
  • Other Species Mouse Btd ELISA KitBovine BTD ELISA KitRat Btd ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human BTD. No significant cross-reactivity or interference between human BTD and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human BTD is typically less than 18.75 muU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human BTD concentration that could be differentiated from zero. It was determined the
  • Intended UseHuman BTD ELISA Kit allows for the in vitro quantitative determination of BTD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BTD. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated BTD. The competitive inhibition reaction is launched between with HRP-conjugated BTD and BTD in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of BTD in the sample. The color development is stopped and the intensity of the color is measured.
  • Human Biotinidase Protein information
  • Uniprot ID BTD_HUMAN
  • Uniprot AC P43251; A6NHF2; B2R865; B4DFX1; B4DLJ9; B7Z7C9; F8W1Q3; Q96EM9;
  • UniGene Hs.444197; Hs.517830;
  • GeneID 686
  • KEGG hsa:686;
  • Human Biotinidase Protein SEQUENCE
  • SEQUENCE 543 AA; 61133 MW; 1A999893A0784944 CRC64;

    MAHAHIQGGR RAKSRFVVCI MSGARSKLAL FLCGCYVVAL GAHTGEESVA

    DHHEAEYYVA AVYEHPSILS LNPLALISRQ EALELMNQNL DIYEQQVMTA

    AQKDVQIIVF PEDGIHGFNF TRTSIYPFLD FMPSPQVVRW NPCLEPHRFN

    DTEVLQRLSC MAIRGDMFLV ANLGTKEPCH SSDPRCPKDG RYQFNTNVVF

    SNNGTLVDRY RKHNLYFEAA FDVPLKVDLI TFDTPFAGRF GIFTCFDILF

    FDPAIRVLRD YKVKHVVYPT AWMNQLPLLA AIEIQKAFAV AFGINVLAAN

    VHHPVLGMTG SGIHTPLESF WYHDMENPKS HLIIAQVAKN PVGLIGAENA

    TGETDPSHSK FLKILSGDPY CEKDAQEVHC DEATKWNVNA PPTFHSEMMY

    DNFTLVPVWG KEGYLHVCSN GLCCYLLYER PTLSKELYAL GVFDGLHTVH

    GTYYIQVCAL VRCGGLGFDT CGQEITEATG IFEFHLWGNF STSYIFPLFL

    TSGMTLEVPD QLGWENDHYF LRKSRLSSGL VTAALYGRLY ERD

  • UCSC uc003cah.5; human. [P43251-1];


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