Human BTD ELISA Kit

Alternative name
Human biotinase ELISA Kit;Human biotinidase ELISA Kit;
Catalog
E004999
species
Human
GeneBTD
Other Species Mouse Btd ELISA Kit Bovine BTD ELISA Kit Rat Btd ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of human BTD. No significant cross-reactivity or interference between human BTD and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human BTD is typically less than 18.75 muU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human BTD concentration that could be differentiated from zero. It was determined the
Intended UseHuman BTD ELISA Kit allows for the in vitro quantitative determination of BTD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BTD. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated BTD. The competitive inhibition reaction is launched between with HRP-conjugated BTD and BTD in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of BTD in the sample. The color development is stopped and the intensity of the color is measured.
Human Biotinidase Protein information
Uniprot ID BTD_HUMAN
Uniprot AC P43251; A6NHF2; B2R865; B4DFX1; B4DLJ9; B7Z7C9; F8W1Q3; Q96EM9;
UniGene Hs.444197; Hs.517830;
GeneID 686
KEGG hsa:686;
Human Biotinidase Protein SEQUENCE
SEQUENCE 543 AA; 61133 MW; 1A999893A0784944 CRC64;
MAHAHIQGGR RAKSRFVVCI MSGARSKLAL FLCGCYVVAL GAHTGEESVA
DHHEAEYYVA AVYEHPSILS LNPLALISRQ EALELMNQNL DIYEQQVMTA
AQKDVQIIVF PEDGIHGFNF TRTSIYPFLD FMPSPQVVRW NPCLEPHRFN
DTEVLQRLSC MAIRGDMFLV ANLGTKEPCH SSDPRCPKDG RYQFNTNVVF
SNNGTLVDRY RKHNLYFEAA FDVPLKVDLI TFDTPFAGRF GIFTCFDILF
FDPAIRVLRD YKVKHVVYPT AWMNQLPLLA AIEIQKAFAV AFGINVLAAN
VHHPVLGMTG SGIHTPLESF WYHDMENPKS HLIIAQVAKN PVGLIGAENA
TGETDPSHSK FLKILSGDPY CEKDAQEVHC DEATKWNVNA PPTFHSEMMY
DNFTLVPVWG KEGYLHVCSN GLCCYLLYER PTLSKELYAL GVFDGLHTVH
GTYYIQVCAL VRCGGLGFDT CGQEITEATG IFEFHLWGNF STSYIFPLFL
TSGMTLEVPD QLGWENDHYF LRKSRLSSGL VTAALYGRLY ERD
UCSC uc003cah.5; human. [P43251-1];

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Category

Human BTD ELISA Kit

Human biotinase ELISA Kit;Human biotinidase ELISA Kit;