Mouse CA ELISA Kit

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  • Catalog
    E049909
  • species
    Mouse
  • GeneCA
  • Standard CurveMouse CA ELISA Kit
  • Other Species Human Ca (2+) ATPase ELISA KitHuman CA 15-3 ELISA KitHuman CA 19-9 ELISA KitHuman CA 242 ELISA KitHuman CA 72-4 ELISA KitHuman CA IX ELISA KitHuman CA-125 ELISA KitHuman CA-19-9 ELISA KitHuman CA 27-29 ELISA KitHuman CA-IgA ELISA KitHuman CA ELISA KitMouse Ca (2+) -ATPase ELISA KitMouse Ca-ATPase ELISA KitMouse CA-1 ELISA KitMouse CA-2 ELISA KitMouse CA-IgA ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse CA ELISA Kit allows for the in vitro quantitative determination of CA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Intended Uses: This CAELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofCAin the sample, thisCAELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusCAconcentration. The concentration ofCAin the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This CAenzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forP450SCC. Standards or samples are then added to the microtiter plate wells andCAif present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofCApresent in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forCAare added to each well to "sandwich" theCAimmobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containCAand enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.



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