Mouse CX43 ELISA Kit

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  • Alternative name

    Gap junction alpha-1 protein ELISA KIT; Connexin-43 ELISA KIT; Cx43 ELISA KIT; Short fin proteingja1 ELISA KIT; cx43 ELISA KIT; shf ELISA KIT; sof ELISA KIT; Cx43 ELISA KIT

  • Catalog
    E049829
  • species
    Mouse
  • GeneCX43
  • Other Species Human CX43 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse CX43. No significant cross-reactivity or interference between Mouse CX43 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse CX43 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.094ng/mL
  • Intended UseMouse CX43 ELISA Kit allows for the in vitro quantitative determination of CX43 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CX43 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CX43. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CX43 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CX43, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CX43. You can calculate the concentration of Mouse CX43 in the samples by comparing the OD of the samples to the standard curve.


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