Human ATF6 ELISA Kit

Alternative name
Human cAMP-dependent transcription factor ATF-6 alpha ELISA Kit;Human Activating transcription factor 6 alpha ELISA Kit;Human ATF6-alpha ELISA Kit;Human ACHM7 ELISA Kit;Human ATF6A ELISA Kit;Human activating transcription factor 6 ELISA Kit;Human cyclic AMP-dependent transcription factor ATF-6 alpha ELISA Kit;
Catalog
E000497
species
Human
GeneATF6
Standard Curve
Other Species Mouse Atf6 ELISA Kit Rat Atf6 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ATF6 ELISA Kit allows for the in vitro quantitative determination of ATF6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description
specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This ATF6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATF6. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY ATF6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATF6 antibody and an ATF6-HRP conjugate. The assay sample and buffer are incubated together with ATF6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATF6 concentration since ATF6 from samples and ATF6-HRP conjugate compete for the anti-ATF6 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATF6 from the sample, fewer sites are left to bind ATF6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATF6 concentration in each sample is interpolated from this standard curve.
Human Processed cyclic AMP-dependent transcription factor ATF-6 alpha Protein information
Uniprot ID ATF6A_HUMAN
Uniprot AC P18850; O15139; Q5VW62; Q6IPB5; Q9UEC9;
UniGene Hs.492740; Hs.617868;
GeneID 22926
KEGG hsa:22926;
Human Processed cyclic AMP-dependent transcription factor ATF-6 alpha Protein SEQUENCE
SEQUENCE 670 AA; 74585 MW; 5EBD08CF4121D41A CRC64;
MGEPAGVAGT MESPFSPGLF HRLDEDWDSA LFAELGYFTD TDELQLEAAN
ETYENNFDNL DFDLDLMPWE SDIWDINNQI CTVKDIKAEP QPLSPASSSY
SVSSPRSVDS YSSTQHVPEE LDLSSSSQMS PLSLYGENSN SLSSAEPLKE
DKPVTGPRNK TENGLTPKKK IQVNSKPSIQ PKPLLLPAAP KTQTNSSVPA
KTIIIQTVPT LMPLAKQQPI ISLQPAPTKG QTVLLSQPTV VQLQAPGVLP
SAQPVLAVAG GVTQLPNHVV NVVPAPSANS PVNGKLSVTK PVLQSTMRNV
GSDIAVLRRQ QRMIKNRESA CQSRKKKKEY MLGLEARLKA ALSENEQLKK
ENGTLKRQLD EVVSENQRLK VPSPKRRVVC VMIVLAFIIL NYGPMSMLEQ
DSRRMNPSVS PANQRRHLLG FSAKEAQDTS DGIIQKNSYR YDHSVSNDKA
LMVLTEEPLL YIPPPPCQPL INTTESLRLN HELRGWVHRH EVERTKSRRM
TNNQQKTRIL QGALEQGSNS QLMAVQYTET TSSISRNSGS ELQVYYASPR
SYQDFFEAIR RRGDTFYVVS FRRDHLLLPA TTHNKTTRPK MSIVLPAINI
NENVINGQDY EVMMQIDCQV MDTRILHIKS SSVPPYLRDQ QRNQTNTFFG
SPPAATEATH VVSTIPESLQ
UCSC uc001gbs.4; human.;

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Human ATF6 ELISA Kit