Mouse CTXII ELISA Kit

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  • Catalog
    E049682
  • species
    Mouse
  • GeneCTXII
  • Standard CurveMouse CTXII ELISA Kit
  • Other Species Human CTXII ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Cross Linked C-Telopeptide Of Type II Collagen (CTXII). No significant cross-reactivity or interference between Cross Linked C-Telopeptide Of Type II Collagen (CTXII) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 4.93pg/mL.
  • Intended UseMouse CTXII ELISA Kit allows for the in vitro quantitative determination of CTXII , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cross Linked C-Telopeptide Of Type II Collagen (CTXII) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cross Linked C-Telopeptide Of Type II Collagen (CTXII) and unlabeled Cross Linked C-Telopeptide Of Type II Collagen (CTXII) (Standards or samples) with the pre-coated antibody specific to Cross Linked C-Telopeptide Of Type II Collagen (CTXII). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cross Linked C-Telopeptide Of Type II Collagen (CTXII) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cross Linked C-Telopeptide Of Type II Collagen (CTXII) in the sample.



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