Intended UseMouse CKMT1A ELISA Kit allows for the in vitro quantitative determination of CKMT1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyCell Biology
Product Description specificalPrinciple of the Assay: CKMT1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CKMT1A antibody and an CKMT1A-HRP conjugate. The assay sample and buffer are incubated together with CKMT1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CKMT1A concentration since CKMT1A from samples and CKMT1A-HRP conjugate compete for the anti-CKMT1A antibody binding site. Since the number of sites is limited, as more sites are occupied by CKMT1A from the sample, fewer sites are left to bind CKMT1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CKMT1A concentration in each sample is interpolated from this standard curve.
No manual or datasheet available for this product.