Sensitivity0.05 ng/mL (mean of 6 independent assays).
Intended UseMouse COR ELISA Kit allows for the in vitro quantitative determination of COR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: For the quantitative detection of Mouse Cortisol (COR) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for COR and Horseradish Peroxidase (HRP) conjugated COR. The competitive inhibition reaction is launched between with HRP labeled COR and unlabeled COR with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of COR in the sample. The color development is stopped and the intensity of the color is measured.