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Mouse Cort ELISA Kit

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  • Alternative name

    Mouse CST ELISA Kit;Mouse PCST ELISA Kit;Mouse cortistatin ELISA Kit;
  • Catalog
    E049540
  • species
    Mouse
  • GeneCort
  • Other Species Human CORT ELISA KitRat Cort ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CORT. No significant cross-reactivity or interference between CORT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CORT and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Cort ELISA Kit allows for the in vitro quantitative determination of Cort , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Principle of the assay: CORT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CORT antibody and an CORT-HRP conjugate. The assay sample and buffer are incubated together with CORT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CORT concentration since CORT from samples and CORT-HRP conjugate compete for the anti-CORT antibody binding site. Since the number of sites is limited, as more sites are occupied by CORT from the sample, fewer sites are left to bind CORT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CORT concentration in each sample is interpolated from this standard curve.
  • Mouse Cortistatin-14 Protein information
  • Uniprot ID CORT_MOUSE
  • Uniprot AC P56469; A2AH76; Q9R1P8;
  • UniGene Mm.6204;
  • GeneID 12854
  • KEGG mmu:12854;
  • Mouse Cortistatin-14 Protein SEQUENCE

  • SEQUENCE 109 AA; 11613 MW; 630D3BDD0488F722 CRC64;

    MMGGRGTGGK WPSAFGLLLL WGVAASALPL ESGPTGQDSV QEATEGRSGL

    LTFLAWWHEW ASQASSSTPV GGGTPGLSKS QERPPPQQPP HLDKKPCKNF

    FWKTFSSCK

  • UCSC uc008vvs.1; mouse.;


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