Mouse Cort ELISA Kit

Alternative name
Mouse CST ELISA Kit;Mouse PCST ELISA Kit;Mouse cortistatin ELISA Kit;
Catalog
E049540
species
Mouse
GeneCort
SpecificityThis assay has high sensitivity and excellent specificity for detection of CORT. No significant cross-reactivity or interference between CORT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CORT and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Cort ELISA Kit allows for the in vitro quantitative determination of Cort , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: CORT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CORT antibody and an CORT-HRP conjugate. The assay sample and buffer are incubated together with CORT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CORT concentration since CORT from samples and CORT-HRP conjugate compete for the anti-CORT antibody binding site. Since the number of sites is limited, as more sites are occupied by CORT from the sample, fewer sites are left to bind CORT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CORT concentration in each sample is interpolated from this standard curve.
Mouse Cortistatin-14 Protein information
Uniprot ID CORT_MOUSE
Uniprot AC P56469; A2AH76; Q9R1P8;
UniGene Mm.6204;
GeneID 12854
KEGG mmu:12854;
Mouse Cortistatin-14 Protein SEQUENCE
SEQUENCE 109 AA; 11613 MW; 630D3BDD0488F722 CRC64;
MMGGRGTGGK WPSAFGLLLL WGVAASALPL ESGPTGQDSV QEATEGRSGL
LTFLAWWHEW ASQASSSTPV GGGTPGLSKS QERPPPQQPP HLDKKPCKNF
FWKTFSSCK
UCSC uc008vvs.1; mouse.;

Mouse Cort ELISA Kit

Mouse CST ELISA Kit;Mouse PCST ELISA Kit;Mouse cortistatin ELISA Kit;

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