Human Dyn ELISA Kit

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  • Catalog
    E004954
  • species
    Human
  • GeneDyn
  • Standard CurveHuman Dyn ELISA Kit
  • Other Species Mouse Dyn ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Big Dynorphin (Dyn). No significant cross-reactivity or interference between Big Dynorphin (Dyn) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 2.36pg/mL.
  • Intended UseHuman Dyn ELISA Kit allows for the in vitro quantitative determination of Dyn , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Big Dynorphin (Dyn) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Big Dynorphin (Dyn) and unlabeled Big Dynorphin (Dyn) (Standards or samples) with the pre-coated antibody specific to Big Dynorphin (Dyn). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Big Dynorphin (Dyn) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Big Dynorphin (Dyn) in the sample.




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