Human NDST1 ELISA Kit

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  • Alternative name

    Human glucosaminyl N-deacetylase/N-sulfotransferase 1 ELISA Kit;Human NDST-1 ELISA Kit;Human N-heparan sulfate sulfotransferase 1 ELISA Kit;Human N-HSST 1 ELISA Kit;Human [Heparan sulfate]-glucosamine N-sulfotransferase 1 ELISA Kit;Human HSNST 1 ELISA Kit;Human HSST ELISA Kit;Human HSST1 ELISA Kit;Human MRT46 ELISA Kit;Human NST1 ELISA Kit;Human N-deacetylase and N-sulfotransferase 1 ELISA Kit;Human bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 ELISA Kit;Human N-Deacetylase-N-sulfotransferase 1 ELISA Kit;Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1 ELISA Kit;Human N-deacetylase/N-sulfotransferase 1 ELISA Kit;Human heparan sulfate-N-deacetylase/N-sulfotransferase ELISA Kit;Human heparan sulfate/heparin GlcNAc N-deacetylase/GlcN N-sulfotransferase 1 ELISA Kit;

  • Catalog
    E004943
  • species
    Human
  • GeneNDST1
  • Standard CurveHuman NDST1 ELISA Kit
  • Other Species Mouse Ndst1 ELISA KitRat Ndst1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of BTA. No significant cross-reactivity or interference between BTA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BTA and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman NDST1 ELISA Kit allows for the in vitro quantitative determination of NDST1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the assay: BTA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BTA antibody and an BTA-HRP conjugate. The assay sample and buffer are incubated together with BTA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BTA concentration since BTA from samples and BTA-HRP conjugate compete for the anti-BTA antibody binding site. Since the number of sites is limited, as more sites are occupied by BTA from the sample, fewer sites are left to bind BTA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BTA concentration in each sample is interpolated from this standard curve.
  • Human Heparan sulfate N-sulfotransferase 1 Protein information
  • Uniprot ID NDST1_HUMAN
  • Uniprot AC P52848; Q96E57;
  • UniGene Hs.222055;
  • GeneID 3340
  • KEGG hsa:3340;
  • Human Heparan sulfate N-sulfotransferase 1 Protein SEQUENCE
  • SEQUENCE 882 AA; 100868 MW; D4B716B84A0BF4C4 CRC64;

    MPALACLRRL CRHVSPQAVL FLLFIFCLFS VFISAYYLYG WKRGLEPSAD

    APEPDCGDPP PVAPSRLLPL KPVQAATPSR TDPLVLVFVE SLYSQLGQEV

    VAILESSRFK YRTEIAPGKG DMPTLTDKGR GRFALIIYEN ILKYVNLDAW

    NRELLDKYCV AYGVGIIGFF KANENSLLSA QLKGFPLFLH SNLGLKDCSI

    NPKSPLLYVT RPSEVEKGVL PGEDWTVFQS NHSTYEPVLL AKTRSSESIP

    HLGADAGLHA ALHATVVQDL GLHDGIQRVL FGNNLNFWLH KLVFVDAVAF

    LTGKRLSLPL DRYILVDIDD IFVGKEGTRM KVEDVKALFD TQNELRAHIP

    NFTFNLGYSG KFFHTGTNAE DAGDDLLLSY VKEFWWFPHM WSHMQPHLFH

    NQSVLAEQMA LNKKFAVEHG IPTDMGYAVA PHHSGVYPVH VQLYEAWKQV

    WSIRVTSTEE YPHLKPARYR RGFIHNGIMV LPRQTCGLFT HTIFYNEYPG

    GSSELDKIIN GGELFLTVLL NPISIFMTHL SNYGNDRLGL YTFKHLVRFL

    HSWTNLRLQT LPPVQLAQKY FQIFSEEKDP LWQDPCEDKR HKDIWSKEKT

    CDRFPKLLII GPQKTGTTAL YLFLGMHPDL SSNYPSSETF EEIQFFNGHN

    YHKGIDWYME FFPIPSNTTS DFYFEKSANY FDSEVAPRRA AALLPKAKVL

    TILINPADRA YSWYQHQRAH DDPVALKYTF HEVITAGSDA SSKLRALQNR

    CLVPGWYATH IERWLSAYHA NQILVLDGKL LRTEPAKVMD MVQKFLGVTN

    TIDYHKTLAF DPKKGFWCQL LEGGKTKCLG KSKGRKYPEM DLDSRAFLKD

    YYRDHNIELS KLLYKMGQTL PTWLREDLQN TR

  • UCSC uc003lsk.4; human. [P52848-1];




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