Mouse C1qtnf7 ELISA Kit

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  • Alternative name

    Mouse Ctrp7 ELISA Kit;Mouse 5530401N20Rik ELISA Kit;Mouse 8430425G24Rik ELISA Kit;Mouse C1q and tumor necrosis factor related protein 7 ELISA Kit;Mouse complement C1q tumor necrosis factor-related protein 7 ELISA Kit;Mouse C1q and tumor necrosis factor related protein 5 ELISA Kit;

  • Catalog
    E049307
  • species
    Mouse
  • GeneC1qtnf7
  • Standard CurveMouse C1qtnf7 ELISA Kit
  • Other Species Human C1QTNF7 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of C1QTNF7. No significant cross-reactivity or interference between C1QTNF7 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C1QTNF7 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse C1qtnf7 ELISA Kit allows for the in vitro quantitative determination of C1qtnf7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: This C1QTNF7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse C1QTNF7. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||C1QTNF7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-C1QTNF7 antibody and an C1QTNF7-HRP conjugate. The assay sample and buffer are incubated together with C1QTNF7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C1QTNF7 concentration since C1QTNF7 from samples and C1QTNF7-HRP conjugate compete for the anti-C1QTNF7 antibody binding site. Since the number of sites is limited, as more sites are occupied by C1QTNF7 from the sample, fewer sites are left to bind C1QTNF7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C1QTNF7 concentration in each sample is interpolated from this standard curve.
  • Mouse Complement C1q tumor necrosis factor-related protein 7 Protein information
  • Uniprot ID C1QT7_MOUSE
  • Uniprot AC Q8BVD7; Q8CHX9;
  • UniGene Mm.275553;
  • GeneID 109323
  • KEGG mmu:109323;
  • Mouse Complement C1q tumor necrosis factor-related protein 7 Protein SEQUENCE
  • SEQUENCE 289 AA; 30517 MW; 45ED4E3C88E92CCB CRC64;

    MIVLLYVTSL AICASGQPRA NQAKGESYSP RYICSIPGLP GPPGPPGANG

    SPGPHGRIGL PGRDGRDGRK GEKGEKGTAG LKGKTGPLGL AGEKGDQGET

    GKKGPIGPEG EKGEVGPAGP PGPKGDRGDQ GDPGLPGVCR CGSIVLKSAF

    SVGITTSYPE ERLPIIFNKV LFNEGEHYNP ATGKFICAFP GIYYFSYDIT

    LANKHLAIGL VHNGQYRIRT FDANTGNHDV ASGSTVIYLQ PEDEVWLEIF

    FNDQNGLFSD PGWADSLFSG FLLYVDTDYL DSISEDDEL

  • UCSC uc008xho.2; mouse.;



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