SpecificityThis assay has high sensitivity and excellent specificity for detection of CoL-2CAV. No significant cross-reactivity or interference between CoL-2CAV and analogues was observed. cross-reactivity detection between CoL-2CAV and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseMouse C2C ELISA Kit allows for the in vitro quantitative determination of C2C , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyCell Biology
Product Description specificalIntended Uses||This CoL-2CAV ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CoL-2CAV. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: CoL-2CAV ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CoL-2CAV antibody and an CoL-2CAV-HRP conjugate. The assay sample and buffer are incubated together with CoL-2CAV-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CoL-2CAV concentration since CoL-2CAV from samples and CoL-2CAV-HRP conjugate compete for the anti-CoL-2CAV antibody binding site. Since the number of sites is limited, as more sites are occupied by CoL-2CAV from the sample, fewer sites are left to bind CoL-2CAV-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CoL-2CAV concentration in each sample is interpolated from this standard curve.